A laser-scanning system for radioisotope-labeled, multi-fluorescent, chemifluorescent and colorimetric imaging

用于放射性同位素标记、多荧光、化学荧光和比色成像的激光扫描系统

• 批准号: 472898-2015
• 负责人: Zimmerly, Steven
• 金额: $ 9.33万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564876
• 关键词: laser; scanning; system; radioisotope; labeled

Funds are requested to purchase a general-purpose, state-of-the-art laser scanning imaging system, to be used by multiple research programs in the Department of Biological Sciences. The instrument is a replacement and upgrade of an existing scanning system that has become inoperable and nonfunctional. The proposed instrument has broad applicability for many research purposes, being capable of detecting radiolabeled molecules through phosphorimaging (e.g., P-32, S-35, C-14), and detecting fluorescence signals with blue, green or red lasers, thereby producing images or quantitating data with high accuracy over five orders of magnitude of signal. Experimental techniques enabled by the instrument include the following: biochemical assays and hybridization blots based on radioactive detection; Southern, northern and western blots based on fluorescence detection, scanning of fluorescence data from glass slides or microtiter plates with a resoution of 10 micrometers; 2D DIGE (differential gel electrophoresis) for proteomic applications; and quantitation of visibly stained gels (Coomassie blue, silver). The eight applying labs have quite diverse research programs in the fields of RNA biology (group II introns and reverse transcriptases), structural biology (viral polymerases), plant metabolism (natural bioproduct chemistry), plant physiology (cell signaling, growth), yeast cell biology (cell wall structure and synthesis), developmental biology (limb development of mice), stem cell biology (germ line differentiation in worm) and cancer biology (breast stem cells). Additional labs in the building and department are expected to utilize the instrument because of its great versatility and applicability for many research purposes.

要求资金购买一种通用，最先进的激光扫描成像系统，该系统将由生物科学系的多个研究计划使用。该仪器是对现有扫描系统的替代和升级，该系统变得无法操作和无功能。该拟议的仪器在许多研究目的中具有广泛的适用性，能够通过磷光成像（例如P-32，S-35，C-14）检测放射标记的分子，并检测具有蓝色，绿色或红色激光器的荧光信号，从而在五级级数的五级级数上产生高精度的图像或定量数据。该仪器启用的实验技术包括以下内容：基于放射性检测的生化测定和杂交印迹；基于荧光检测的南部，北部和蛋白质印迹，从载玻片或微量滴定板的荧光数据扫描，均值为10微米；用于蛋白质组学应用的2D DIGE（差异凝胶电泳）；以及明显染色的凝胶（Coomassie Blue，银）的定量。 The eight applying labs have quite diverse research programs in the fields of RNA biology (group II introns and reverse transcriptases), structural biology (viral polymerases), plant metabolism (natural bioproduct chemistry), plant physiology (cell signaling, growth), yeast cell biology (cell wall structure and synthesis), developmental biology (limb development of mice), stem cell biology (germ line differentiation in worm) and cancer生物学（乳房干细胞）。由于其出色的多功能性和适用性用于许多研究目的，因此预计建筑物和部门的其他实验室将使用该工具。