Genomic and proteomic insights into glaucophyte plastids

对灰藻质体的基因组和蛋白质组学见解

• 批准号: 402421-2011
• 负责人: ReyesPrieto, Adrian
• 金额: $ 3.28万
• 依托单位: University of New Brunswick
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=550915
• 关键词: Genomic; proteomic; insights; into; glaucophyte

Photosynthesis is a central physiological process that accounts for most of primary production and oxygen generation in the planet. Photosynthesis evolved in cyanobacteria around three billion years ago. Two billion years after this event some cyanobacteria were recruited via intracellular symbiosis (endosymbiosis) by certain eukaryote cells and evolved into the plastids of algae and plants. It has been hypothesized that extant algae (glaucophytes, red and green algae, including land plants) bearing plastids surrounded by two membranes, and formally named Plantae, are descended from common ancestors harboring plastids derived directly from endosymbiotic cyanobacteria (primary plastids). Testing this hypothesis of common origin of Plantae is critical to understand the emergence of the first photosynthetic eukaryotes. One avenue to study this hypothesis is through comparative studies of genetic and protein repertoires from different Plantae lineages. Green algae and land plants have been thoroughly studied in this respect, but this is not the case for red algae and glaucophytes. My research investigates genomic and functional aspects of plastids from glaucophytes, which likely represent the earliest diverging lineage within Plantae. Thus, the study of glaucophytes is important for understanding the origin and diversification of Plantae. My project comprises three specific objectives. 1) Comparative transcriptomics. Pools of expressed genes (mRNAs) from different glaucophytes will be sequenced and profiled. We will study encoded proteins with emphasis on the plastid-targeted component. 2) Plastid genomics. We will sequence plastid genomes of diverse glaucophyte species. We will analyze sequence data to explore gene content, genome architecture and diversity within the group. 3) Plastid proteomics. We will identify plastid protein collections of diverse glaucophytes. We will compare these data with corresponding datasets from other Plantae and our transcriptome data. We will identify both plastid protein core components shared between Plantae and unique protein traits. In toto, data from the three objectives will test both the presumed common origin of Plantae plastids and the early divergence of glaucophytes.

光合作用是一个中心的生理过程，它是地球中主要产量和氧气产生的大部分。光合作用在大约三十亿年前的蓝细菌中演变。这次事件发生后的20亿年，通过某些真核病细胞通过细胞内共生（内共生）募集了一些蓝细菌，并演变成藻类和植物的质体。已经假设，现存的藻类（绿木菌，红色和绿藻，包括土地植物）载有质体，这些藻类被两个膜包围，并正式命名为植物学，是直接来自内生物生物囊肿的质体的共同祖先的后代。检验植物学共同起源的假设对于了解第一个光合真核生物的出现至关重要。研究这一假设的一个途径是通过对不同植物谱系的遗传和蛋白质谱的比较研究。在这方面已经对绿藻和土地植物进行了彻底的研究，但是红藻和绿木菌的情况并非如此。我的研究研究了质体的质体基因组和功能方面，这可能代表了植物学中最早的谱系。因此，青光眼研究对于理解植物学的起源和多样化很重要。我的项目包括三个特定目标。 1）比较转录组学。将测序并构造来自不同青光眼植物的表达基因（mRNA）的池。我们将研究编码的蛋白质，重点是塑料成分。 2）质体基因组学。我们将对各种青光眼物种的质体基因组进行序列。我们将分析序列数据，以探索小组内的基因含量，基因组结构和多样性。 3）质体蛋白质组学。我们将确定各种青光眼植物的质体蛋白收集。我们将将这些数据与来自其他植物学的相应数据和我们的转录组数据进行比较。我们将确定植物性和独特蛋白质性状之间共享的质体蛋白质核心成分。在Toto中，来自三个目标的数据将同时检验植物质体的普通起源和青光眼的早期差异。