Functional analysis of the complete MADS-box gene family in the moss, physcomitrella patens

苔藓、小立碗藓中完整 MADS-box 基因家族的功能分析

• 批准号: 2982-2008
• 负责人: Ashton, Neil
• 金额: $ 1.83万
• 依托单位: University of Regina
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=388683
• 关键词: Functional; analysis; complete; MADS; box

MADS-box genes are indispensable elements in the genetic programming of pattern formation, including the segmental organisation of angiosperm flowers, in all seed plants. Thus, MADS-box genes are best known for their roles in regulating the transition of shoot apical meristems into inflorescence and floral meristems and in the determination of floral organ (sepal, petal, stamen and carpel) identities. They have also been discovered in non-flowering plants including conifers, Gnetum, ferns, a clubmoss and a moss. In a few cases, it has been shown that specific non-flowering plant MADS-box genes display similar expression patterns to distinct subsets of flowering plant MADS-box genes. Therefore, discovery of the non-flowering plant genes has raised the intriguing possibility that mechanisms governing the differentiation of reproductive structures, and other important developmental transitions, in higher and at least some lower plants are fundamentally similar and evolutionarily conserved. To obtain clues about the ancestral functions of genes that are now involved in regulating specialised developmental processes such as the generation of flowers, the research proposed here will determine the roles and expression patterns of the entire complement of MADS-box genes of the moss, Physcomitrella patens. This plant model system offers unique opportunities for these kinds of study because its entire genome has been sequenced and it is amenable to sophisticated genetic analysis. The results obtained, when combined with information about this gene family in other plants, should provide a more profound comprehension of the evolution of the MADS-box multigene family and its functions as well as of the evolution of the various major land plant groups and structures, such as angiosperm flowers, which define each of them.

在所有种子植物中，MADS-Box基因是模式形成的基因编程，包括被子植物花的分段组织。 因此，MADS-box基因以其在调节芽顶分生组织过渡到花序和花卉分生组织以及花卉器官（Sepal，Petal，雄蕊，雄蕊和地球）的角色方面的作用而闻名。 它们也在包括针叶树，gnetum，蕨类植物，俱乐部和苔藓等非开花植物中发现。 在少数情况下，已经表明，特定的非开花植物MADS-BOX基因显示出与开花植物Mads-Box基因不同子集相似的表达模式。 因此，发现非花费植物基因的发现增加了一种有趣的可能性，即管理生殖结构分化的机制以及在较高和至少一些较低的植物中的其他重要发育过渡的可能性在根本上是相似的，并且在进化上是保守的。 为了获得有关现在参与调节专业发育过程（例如花产生）基因祖先功能的线索，此处提出的研究将确定苔藓盒，Phanscomitrella Patens的MADS-box基因的整个补体的作用和表达模式。 该植物模型系统为此类研究提供了独特的机会，因为它的整个基因组已经测序，并且可以接受复杂的遗传分析。 当与其他植物中有关该基因家族的信息结合使用时，应更深刻地理解MADS-Box多基因家族及其功能的演变以及各种主要土地植物群和结构的演变，例如nabiosperm Flowers，这些花朵定义了它们。