The role of tandem tudor domain proteins in RNAi

串联 tudor 结构域蛋白在 RNAi 中的作用

• 批准号: 341457-2007
• 负责人: Duchaîne, Thomas
• 金额: $ 2.05万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=364174
• 关键词: role; tandem; tudor; domain; proteins

RNA-mediated interference, or RNAi, is the specific shut down of a gene's expression by the exposure of cells to double-stranded RNA, or to its derivatives. This phenomenon has had a deep impact on biological sciences in the recent years, primarily for its use to experimentally examine the outcome of the loss of any gene's function in experiments. In the most recent years it is becoming clear that RNAi also serves numerous physiological purposes. Through a diverse variety of related processes, it can profoundly influence gene regulatory networks. In the current research proposal, we put forward an integrated strategy to address the molecular basis for one of these phenomena termed endogenous RNAi (or endoRNAi). EndoRNAi modifies gene expression by converging its silencing signals on chromatin (the physiological architechtures of DNA). We will examine the function of two related proteins required for endoRNAi, which encode tandem tudor domains. Our previous work supports a model wherein these proteins direct the RNAi machinery to reinforce the silenced state of genes. The current proposal will result in a better understanding of the biological functions of RNAi, of the underlying mechanisms, and further elucidate the functions of the tudor domains in gene regulation.

RNA 介导的干扰或 RNAi 是通过将细胞暴露于双链 RNA 或其衍生物来特异性关闭基因表达。近年来，这种现象对生物科学产生了深远的影响，主要是因为它可以用来通过实验检查任何基因功能丧失的结果。近年来，人们越来越清楚 RNAi 还具有多种生理目的。通过各种相关过程，它可以深刻地影响基因调控网络。在当前的研究提案中，我们提出了一种综合策略来解决这些现象之一的分子基础，称为内源性RNAi（或endoRNAi）。 EndoRNAi 通过将沉默信号汇聚到染色质（DNA 的生理结构）上来修改基因表达。我们将检查 endRNAi 所需的两种相关蛋白的功能，它们编码串联 tudor 结构域。我们之前的工作支持一个模型，其中这些蛋白质指导 RNAi 机制来强化基因的沉默状态。目前的提议将有助于更好地理解 RNAi 的生物学功能及其潜在机制，并进一步阐明 tudor 结构域在基因调控中的功能。