Translational Study of Vocal Exercise Dose-response

发声运动剂量反应的转化研究

基本信息

批准号：
9362149
负责人：
Aaron Matthew Johnson
金额：
$ 14.06万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-04-01 至 2020-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9362149
关键词：
Acoustics Affect Aging Animal Model Animals Area Behavioral Clinical Clinical Trials Design Complement Control Groups Dose Elderly Endoscopy Exercise Exercise Physiology Goals Grant Growth Health Home environment Human Hypertrophy Intervention K-Series Research Career Programs Kinesiology Laboratories Laryngeal muscle structure Larynx Lead Limb structure Loudness Magnetic Resonance Imaging Measures Mentors Mentorship Morphology Muscle Muscle Fibers Muscle function Muscular Atrophy Neurologic Neuromuscular Junction Operant Conditioning Outcome Outcome Measure Paralysed Peer Review Phonation Phosphorylation Preparation Proteins Publications Rattus Recording of previous events Reporting Research Research Methodology Research Project Grants Resolution Rotation Signal Transduction Speed Structure Testing Thyroarytenoid Muscle Training Translational Research Translations Ultrasonics Voice Voice Disorders Woman Work Writing aged base career development clinical care design dosage evidence base exercise intervention functional improvement improved innovation kinematics men muscle form muscle hypertrophy muscle strength muscular structure neuromuscular neuromuscular system normal aging novel post intervention primary outcome programs resistance exercise response senescence skeletal muscle growth strength training success translational approach translational study vocal cord vocalization voice therapy young adult

项目摘要

DESCRIPTION (provided by applicant): Voice therapy is essential for improving vocal function across a range of voice disorders. For hypofunctional voice disorders associated with aging, voice therapy can improve vocal quality and stamina using vocal exercises that engage the intrinsic laryngeal musculature, but the mechanisms underlying these improvements are unknown. Putative mechanisms are laryngeal muscle growth (hypertrophy) and increased strength based on findings from limb exercise studies that report improvement of these factors along with functional improvements following progressive resistance exercise. Understanding how vocal exercise impacts laryngeal muscle structure and function is critical for determining the most appropriate dose of voice therapy. The overall objective of this research is to determine the dose-response relationship of vocal exercise to both vocal function and laryngeal muscle hypertrophic signaling and growth. Our central hypothesis is that only a high dose of vocal exercise based on progressive resistance training principles will result in beneficial changes in both vocal function and laryngeal muscle hypertrophy in the aging voice. We will test this hypothesis using a translational approach that includes: (1) an innovative behavioral animal model, in which rats will be trained to increase the number of ultrasonic vocalizations, allowing us to investigate neuromuscular mechanisms that are not possible to study in humans, including changes in muscle fiber size and type, neuromuscular junctions, and hypertrophic signaling regulating skeletal muscle growth in response to exercise; and (2) vocal training in humans incorporating traditional clinical measures of vocal function with novel magnetic resonance imaging of laryngeal morphology. This research has two specific aims: (1) To determine the laryngeal hypertrophic neuromuscular response to vocal exercise dosage in young adult and old rats; and (2) To determine the vocal exercise dose-response of laryngeal structure and function in aged men and women. The candidate for this career development award has strong training in the clinical care of voice disorders and in using translational research methods to investigate laryngeal neuromuscular mechanisms. Additional training and mentorship will allow the candidate to develop expertise in muscle exercise physiology and human clinical trial design and implementation. The assembled mentoring team has research expertise in these areas as well as a history of successful and effective mentoring to guide the candidate through the research project described above and the proposed career development activities, including formal coursework in kinesiology, rotations in the mentors' laboratories, and mentored grant writing and peer-review publication preparation. The outcome of this proposal will not only be an understanding of how vocal exercise dose impacts vocal function and structure, but will also help the candidate launch a productive and independent research program to continue investigating laryngeal neuromuscular mechanisms underlying functional changes resulting from voice therapy and vocal training.

描述（由申请人提供）：声音治疗对于改善一系列声音障碍的声音功能至关重要，对于与衰老相关的功能低下的声音障碍，声音治疗可以通过使用内在喉部肌肉组织的声音练习来改善声音质量和耐力。这些改善背后的机制尚不清楚，根据肢体运动研究的结果，推测的机制是喉部肌肉生长（肥大）和力量增加，这些研究报告了这些因素的改善以及渐进性阻力运动后的功能改善。影响喉部肌肉结构和功能对于确定最合适的发声治疗剂量至关重要。本研究的总体目标是确定发声运动对发声功能和喉部肌肉肥大信号传导和生长的剂量反应关系。的观点是，只有基于渐进阻力训练原则的高剂量发声练习才会对老化声音中的发声功能和喉部肌肉肥大产生有益的变化，我们将使用包括以下内容的转化方法来检验这一假设：（1）一种创新的方法。行为的动物模型，其中将训练大鼠增加超声波发声的次数，使我们能够研究无法在人类身上研究的神经肌肉机制，包括肌纤维大小和类型、神经肌肉接头以及调节骨骼肌的肥大信号的变化运动响应的生长；（2）将传统的发声功能临床测量与喉部形态的新型磁共振成像相结合的人类发声训练本研究有两个具体目的：（1）确定喉部肥大。青年和老年大鼠对发声运动剂量的神经肌肉反应；以及（2）确定老年男性和女性喉部结构和功能的发声运动剂量反应。额外的培训和指导将使候选人能够发展肌肉运动生理学和人体临床试验设计和实施方面的专业知识。作为成功和有效指导候选人完成上述研究项目和拟议的职业发展活动的历史，包括运动机能学的正式课程、导师实验室的轮换以及指导的资助写作和同行评审出版物的准备。该提案的目的不仅是了解发声运动剂量如何影响发声功能和结构，还将帮助候选人启动一项富有成效的独立研究计划，以继续研究发声治疗和发声训练引起的功能变化背后的喉部神经肌肉机制。