A two-photon ophthalmoscope for human retinal imaging and functional testing

用于人类视网膜成像和功能测试的双光子检眼镜

• 批准号: 9059094
• 负责人: Krzysztof Palczewski
• 金额: $ 69.95万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9059094
• 关键词: Address; Affect; Age related macular degeneration; American; Americas; Area; Biochemical; Biochemistry; Cell Survival; Chills; Clinical; Clinical assessments; Collaborations; Computer software; Custom; Data; Dependence; Detection; Development; Diagnosis; Diagnostic; Diagnostic Imaging; Disease; Early Diagnosis; Electronics; Electrophysiology (science); Examinations and Diagnoses; Eye; Family suidae; Financial compensation; Fluorescence; Foundations; Functional Imaging; Funding; Genetically Engineered Mouse; Goals; Health; Human; Image; Imagery; Imaging technology; Individual; Institutes; Investigational Therapies; Knowledge; Lasers; Lesion; Light; Lighting; Mammals; Measurement; Measures; Metabolic; Metabolism; Monitor; Monkeys; Mus; Ophthalmology; Ophthalmoscopes; Ophthalmoscopy; Optical Coherence Tomography; Optics; Outcome; Pharmacological Treatment; Phototransduction; Pigments; Research; Resolution; Resources; Retina; Retinal; Retinal Diseases; Retinal Pigments; Retinoids; Risk; Safety; Scanning; Staging; Stem cells; Structure; Technology; Testing; Tissues; Treatment Effectiveness; Vertebrate Photoreceptors; Vision; Visual system structure; absorption; adaptive optics; adduct; base; cellular imaging; chromophore; combat; detector; drug discovery; fluorophore; follow-up; human subject; improved functioning; in vivo; in vivo imaging; instrument; light intensity; mouse model; new technology; novel; novel diagnostics; programs; prototype; relating to nervous system; research study; response; success; tissue regeneration; tool; two-photon; visual cycle

 DESCRIPTION (provided by applicant): One of the stated goals of the NEI Audacious Goal Initiative in addressing the technical needs and opportunities for imaging the visual system (U01) is "to develop new technology for the non-invasive measurement of retina function in vivo as related to natural vision, including visualization of metabolism at the cellular level and electrical activities". Based on strong newly acquired preliminary data, this application proposes the development of a new instrument that can image the retina in exceptional detail that together with two highly sensitive related tests can be used to evaluate retinal function. The specific aims are: (A) with a mouse two-photon ophthalmoscope (TPO), develop a novel diagnostic paradigm used to assess two functional aspects of vision: the metabolic flow of retinoids through the retinoid cycle and visual sensitivity. Along with mass spectrometric analyses, we will define critical intersections between images, structure, function and toxic molecules that affect the retina and identify potential therapies that preserve this neural tissue; and (B) develop and test a TPO imaging and diagnostic tool in humans. Initial tests will be done in a surrogate monkey/pig eye and then with the knowledge gained, we will image the eyes of individuals with advanced stages of blinding retinal diseases. Despite its' `audacity', this proposal can realistically be completed during the funding cycle. The scientific questions and interim milestones required to make this program successful are presented herein.

 描述（由申请人提供）：NEI Audacious Goal Initiative 在解决视觉系统成像技术需求和机会 (U01) 方面的既定目标之一是“开发用于体内视网膜功能非侵入性测量的新技术”与自然视觉相关，包括在细胞水平上高度代谢和电活动的可视化“。基于新获得的强大初步数据，该应用建议开发一种新仪器，可以对视网膜进行极其详细的成像，并结合两个敏感的相关测试可以用于评估视网膜功能的具体目标是：（A）使用小鼠双光子检眼镜（TPO）开发一种新的诊断范例，用于评估视觉的两个功能方面：类维生素A循环中的代谢流和视觉敏感性。随着质谱分析，我们将定义影响视网膜的图像、结构、功能和有毒分子之间的关键交叉点，并确定保护这种神经组织的潜在疗法； (B) 在人体中开发和测试 TPO 成像和诊断工具。初步测试将在替代猴/猪的眼睛中进行，然后根据所获得的知识，我们将对患有晚期致盲性视网膜疾病的个体的眼睛进行成像。尽管其“大胆”，但该提案实际上可以在资助周期内完成。本文介绍了使该计划成功所需的科学问题和临时里程碑。