microRNA gene networks regulating responses to environment

microRNA基因网络调节对环境的反应

• 批准号: 8851625
• 负责人: Victor Ambros
• 金额: $ 64.56万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8851625
• 关键词: Animals; Biological Models; Caenorhabditis elegans; Data; Development; Developmental Process; Diabetes Mellitus; Diet; Disease; Environment; Equilibrium; Family; Feedback; Food; Gene Expression; Gene Expression Regulation; Gene Targeting; Genes; Genetic; Genetic study; Goals; Health; Homologous Gene; Human; Investigation; Link; Malignant Neoplasms; Mediating; Messenger RNA; Metabolic stress; Metabolic syndrome; MicroRNAs; Modeling; Molecular Genetics; Natural Immunity; Nutritional; Organism; Pathway interactions; Physiological; Physiological Processes; Play; Process; Property; Regulator Genes; Role; Signal Transduction; Source; Stress; Time; Work; abstracting; biological adaptation to stress; design; environmental change; genetic approach; genome-wide; genome-wide analysis; novel; pathogen; programs; research study; response

DESCRIPTION (provided by applicant): Abstract The broad goal of this work is to use the C. elegans model system to understand the genetic and molecular mechanisms underlying how microRNAs mediate organismal responses to the nutitional and pathogenic properties of an animal's diet, and help confer developmental and physiological robustness against environmental change. There are more than 150 distinct microRNA genes in C. elegans, including more than 20 families of microRNAs with homologs in humans. Although it is clear that microRNAs play important roles in a wide spectrum of developmental and phsyiological processes in all animals, the functions of these microRNAs are not well understood in detail. Nor is it well understood how microRNA gene expression is regulated transcriptionally or post-transcriptionally by developmental and physiological signals. Molecular and genetic approaches will be employed to produce a comprehensive, genome-wide analysis of microRNA gene regulatory networks in C.elegans, with an emphasis on identifying microRNAs that mediate responses to the nutritional and pathogenic qualities of the animals' bacterial diet diet. In depth functional analysis will be conducted for a set of specific evolutionarily conserved microRNAs that are known to act in pathways that confer developmental and physiological robustness in the context of changing dietery conditions. .

描述（由申请人提供）： 摘要 这项工作的总体目标是使用线虫模型系统来了解 microRNA 如何介导生物体对动物饮食的营养和致病特性的反应的遗传和分子机制，并帮助赋予针对环境变化的发育和生理鲁棒性。 。秀丽隐杆线虫中有超过 150 个不同的 microRNA 基因，其中包括 20 多个与人类同源的 microRNA 家族。尽管很明显 microRNA 在所有动物的广泛发育和生理过程中发挥着重要作用，但这些 microRNA 的功能尚不清楚。 microRNA 基因表达如何通过发育和生理信号进行转录或转录后调节也尚不清楚。将采用分子和遗传学方法对线虫中的 microRNA 基因调控网络进行全面的全基因组分析，重点是鉴定介导对动物细菌饮食的营养和致病品质的反应的 microRNA。将对一组特定的进化上保守的 microRNA 进行深入的功能分析，这些 microRNA 已知在饮食条件变化的情况下在赋予发育和生理鲁棒性的途径中发挥作用。 。