Histomics

组织学

• 批准号: 8937284
• 负责人: EDWARD M LEVINE
• 金额: $ 16.51万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8937284
• 关键词: Area; Award; Basic Science; Collaborations; Cryopreservation; Cryoultramicrotomy; Disease; Evolution; Faculty; Genetic; Genotype; Glaucoma; Grant; Individual; Laboratories; Mus; Paraffin Embedding; Research; Research Personnel; Resources; Retinal Degeneration; Services; System; Technical Expertise; Tissues; Training; Translational Research; Universities; Utah; Vision research; developmental disease; eye center; immunocytochemistry; working group

Project Summary The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 15 investigators holding 21 NEI R01 awards. The Histomics Module derives from the strengths in mouse genetics at the Moran Eye Center and the high demand for tissue profiling. Almost every JMEC laboratory has need of high quality “histomics” (cryosectioning, paratoming, genotyping, immunocytochemistry, PCR, ISH). Specifically the Histomics module provides: · High-quality cryotomy resources through two professionally maintained Leica CM3050S systems · Paraffin embedding and section services via a new Leica Paratome · PCR and genotyping services · Mouse line cryopreservation services

项目概要 该视觉研究核心 (VRC) 的总体目标是提供： · 获取个人 R01 奖项范围之外的资源 · 获得单一实验室范围之外的技术专业知识 · 员工培训以消除高效转化研究和协作的障碍 VRC 支持的研究领域涵盖视网膜变性的分析和治疗、 发育障碍、青光眼等疾病，以及一系列前沿基础疾病 我们实施了四个资源模块，以继续自然发展。 该研究小组如何协同工作，为 15 名获得 21 个 NEI R01 奖项的研究人员提供服务。 组织学模块源自莫兰眼科中心的小鼠遗传学优势和 对组织分析的高需求 几乎每个 JMEC 实验室都需要高质量的“组织组学”。 （冷冻切片、切片、基因分型、免疫细胞化学、PCR、ISH）。 具体来说，组织学模块提供： · 通过两台专业维护的 Leica CM3050S 系统提供高质量的冷冻切片资源 · 通过新型 Leica Paratome 进行石蜡包埋和切片服务 · PCR和基因分型服务 · 小鼠线冻存服务