Molecular Basis for Transmembrane Conduction & Signaling

跨膜传导的分子基础

• 批准号: 8786562
• 负责人: Robert M Stroud
• 金额: $ 55.85万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-04-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8786562
• 关键词: Acetylcholine; Agonist; Ammonia; Anions; Anopheles Genus; Antibodies; Antimalarials; Binding; Biological Assay; Blood; C-terminal; Cells; Cholinergic Receptors; Complex; Crystallization; Culicidae; Development; Disease; Diuresis; Electric Fish; Electrostatics; Erythrocytes; Fab Immunoglobulins; Family; Family member; Female; Future; Genes; Glycerol; Goals; Helicobacter pylori; Histidine; Homologous Gene; Human; Hydrogen Bonding; Length; Libraries; Lung; Malaria; Membrane; Membrane Proteins; Methods; Microbiology; Molecular; Nature; Neurons; Nitrates; Permeability; Physiological; Property; Proteins; Pseudomonas aeruginosa; Rattus; Resolution; Rh Factors; Signal Transduction; Structure; Study models; Technology; Time; Tissues; Urea; Ursidae Family; Water; Xenopus oocyte; aquaporin 3; aquaporin-6; base; desensitization; drug discovery; feeding; kidney cell; ligand gated channel; light scattering; molecular dynamics; patch clamp; protein complex; proteoliposomes; receptor; receptor expression; reconstitution; screening; therapeutic target; transmission process; urea transporter; water channel

We have made substantial progress on several of the Specific Aims proposed in 2006 and further focused future directions toward the challenging long-time aim of understanding eukaryotic channel gating by ligands, particularly in the Acetylcholine Receptor (AcChR). A major subtext has always been to attempt to define structures of eukaryotic and human proteins because of their importance for drug discovery. Towards this goal, we determined two of the first four structures for human membrane proteins, using expression in human cultured HEK293S cells.

我们在 2006 年提出的几个具体目标上取得了实质性进展，并进一步将未来的方向集中在理解配体的真核通道门控这一具有挑战性的长期目标，特别是在乙酰胆碱受体 (AcChR) 中。一个主要的潜台词一直是试图定义真核和人类蛋白质的结构，因为它们对药物发现的重要性。为了实现这一目标，我们利用人类培养的 HEK293S 细胞中的表达确定了人类膜蛋白的前四种结构中的两种。