Mouse Core

鼠标核心

• 批准号: 8236983
• 负责人: ADELINE M HAJJAR
• 金额: $ 33.21万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8236983
• 关键词: Adjuvant; Affect; Alkaline Phosphatase; Breeding; Budgets; Burkholderia; Centers of Research Excellence; Communicable Diseases; Congenic Mice; Emerging Communicable Diseases; Funding; Generations; Genes; Genetic; Genotype; Human; Idaho; Immune; Immunity; Infection; Intervention; Intestines; Lipid A; Modeling; Mus; Mutant Strains Mice; Natural Immunity; Oregon; Pathogenesis; Plague; Pneumonia; Research Personnel; Resources; Role; Speed; TLR4 gene; Therapeutic Effect; Transgenic Mice; Universities; Variant; Washington; acquired immunity; biodefense; congenic; mouse model; mutant; pathogen; prophylactic; response

The overall objective of this core is to generate, breed, genotype and provide genetically modified mice to investigators of the NWRCE. The unique resources developed and managed by this core and the increased needs and anticipated utilization of these resources motivated the creation of a separate transgenic mouse core for this competitive renewal. The Specific Aims of this core are: Aim 1) Advise investigators of the RCE on appropriate use of mouse models for their studies. Aim 2) Breeding, husbandry, and genotyping of existing mutant mouse lines and generation of new compound genetic mutants through intercrossing and genotyping of existing mutant mouse lines. Aim 3) Generation of new genetically modified mice. Aim 4) Rapid generation of congenic mice from mice on mixed genetic backgrounds using a 'speed congenics' approach.

该核心的总体目标是生成、繁殖、基因分型并提供转基因小鼠 NWRCE 的调查员。该核心开发和管理的独特资源以及增加的 这些资源的需求和预期利用激发了单独转基因小鼠的创建 这种竞争更新的核心。该核心的具体目标是： 目标 1) 为 RCE 的研究人员提供有关在研究中适当使用小鼠模型的建议。 目标 2) 现有突变小鼠品系的选育、饲养和基因分型以及新小鼠品系的产生 通过现有突变小鼠品系的交叉和基因分型来复合遗传突变体。 目标 3) 产生新的转基因小鼠。 目标 4) 使用“速度”从混合遗传背景的小鼠中快速生成同源小鼠 congenics 的方法。