STRUCTURAL STUDIES OF MEMBRANE PROTEINS

膜蛋白的结构研究

• 批准号: 8169970
• 负责人: T M Iverson
• 金额: $ 0.3万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169970
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Crystallization; Feedback; Funding; Grant; Growth; Home environment; Institution; Integral Membrane Protein; Membrane Proteins; Process; Proteins; Research; Research Personnel; Resolution; Resources; Screening procedure; Source; Staging; Structure; Synchrotrons; Time; United States National Institutes of Health; Visual; base; frontier; protein structure; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Membrane protein structure determination remains a frontier in structural biology. Growth of diffraction quality crystals is a bottleneck, while growth of crystals diffracting to high-resolution is rare. Using a new strategy, we grew crystals of four integral-membrane proteins, which are currently at different stages of crystallographic characterization. Once crystals of membrane proteins have been grown, the screening process is more involved than for soluble proteins. Often, little diffraction is observed at the home generator, and since the optimization of crystallization conditions is based on feedback from diffraction rather than from the visual quality of the crystals, much more synchrotron time is required to determine each structure.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 膜蛋白结构测定仍然是结构生物学的前沿。衍射质量晶体的生长是一个瓶颈，而高分辨率衍射晶体的生长却很少见。使用一种新策略，我们生长了四种整合膜蛋白的晶体，这些蛋白目前处于晶体学表征的不同阶段。一旦膜蛋白晶体生长出来，筛选过程比可溶性蛋白的筛选过程更加复杂。通常，在家用发生器中几乎观察不到衍射，并且由于结晶条件的优化是基于衍射的反馈而不是晶体的视觉质量，因此需要更多的同步加速器时间来确定每个结构。