STRUCTURAL GENOMICS OF CYTOCHROME P450S

细胞色素 P450S 的结构基因组学

• 批准号: 8170100
• 负责人: Charles David Stout
• 金额: $ 0.78万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170100
• 关键词: Active Sites; Address; Biodiversity; Biology; Bispecific Antibody 2B1; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Cytochrome P450; Cytochromes; Enzymes; Fostering; Funding; Grant; Human Genome; Institution; Mitochondria; Productivity; Protein Conformation; Reaction; Research; Research Personnel; Research Project Grants; Resources; Source; Specificity; Structure; Time; United States National Institutes of Health; Xenobiotics; catalyst; insight; programs; research study; structural genomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this program proposal extension, SSRL beam time is requested to continue experiments on the crystal structures of microsomal and mitochondrial cytochrome P450s, including P450s 1A2, 2A6, 2A13, 2B1, 2B4, 2C8, 2C9, 2C19, 3A4, Cyp24A1, Cyp46A1, Cyp73A5, and Cyp98A3. Together, six NIH supported research projects, focused on P450 structure and function, are represented in these crystallographic experiments. Access to SSRL beam lines allows a high throughput approach, greatly enhancing productivity, and fostering collaboration and insight among the investigators. Cytochrome P450s are universal catalysts for monoxygenation reactions and are widespread in biology, including ~60 distinct enzymes in the human genome. These combined studies represent a structural genomics approach, addressing the range of protein conformations and active site interactions required for activity and specificity toward an immense diversity of biological substrates and xenobiotic molecules.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在此计划提案扩展中，要求 SSRL 光束时间继续对微粒体和线粒体细胞色素 P450 的晶体结构进行实验，包括 P450 1A2、2A6、2A13、2B1、2B4、2C8、2C9、2C19、3A4、Cyp24A1、Cyp46A1、Cyp73A5 ， 和Cyp98A3。这些晶体学实验代表了 NIH 支持的六个研究项目，重点关注 P450 结构和功能。使用 SSRL 光束线可以实现高通量方法，大大提高生产力，并促进研究人员之间的协作和洞察力。细胞色素 P450 是单氧合反应的通用催化剂，在生物学中广泛存在，包括人类基因组中约 60 种不同的酶。这些综合研究代表了一种结构基因组学方法，解决了对生物底物和外源分子的巨大多样性的活性和特异性所需的蛋白质构象和活性位点相互作用的范围。