MR Monitoring of Engineered Tissues

工程组织的 MR 监测

• 批准号: 7807899
• 负责人: RICHARD L MAGIN
• 金额: $ 36.36万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7807899
• 关键词: Animal Model; Animals; Binding; Biochemical; Biology; Biomedical Engineering; Biopsy; Bone Growth; Cartilage; Cells; Chicago; Chondrogenesis; Clinical; Collagen; Data; Defect; Development; Diffusion; Disease; Elasticity; Engineering; Enzyme-Linked Immunosorbent Assay; Extracellular Matrix; Fatty acid glycerol esters; Feedback; Future; GAG Gene; Goals; Growth; Histological Techniques; Human; Image; Imagery; Imaging Techniques; Implant; In Vitro; Injury; Knowledge; Length; Magnetic Resonance Elastography; Magnetic Resonance Imaging; Maps; Measures; Mechanics; Medicine; Mesenchymal Stem Cells; Methods; Microscopic; Monitor; Morphogenesis; Morphology; Mus; Natural regeneration; Optics; Organ; Osteogenesis; Pathway interactions; Perfusion; Phase; Procedures; Process; Property; Prosthesis; Proteoglycan; Protocols documentation; Regenerative Medicine; Relaxation; Resolution; Sampling; Scientist; Staging; Structure; System; Techniques; Temperature; Time; Tissue Differentiation; Tissue Engineering; Tissue Grafts; Tissue Sample; Tissues; Universities; Viscosity; Water; Weight; Work; base; bone; cancer therapy; density; design; imaging modality; implantation; in vivo; lipid biosynthesis; mouse model; physical property; public health relevance; restoration; scaffold; success; tissue regeneration; tool; water diffusion

DESCRIPTION (provided by applicant): Tissue engineering and regenerative medicine (TE/RM) is an evolving interdisciplinary field that integrates engineering with biology and medicine for the development of functional tissues and organs. Monitoring of tissues in vitro prior to implantation and assessment of tissues in vivo during development are both essential in order to optimize the regeneration of tissue and the restoration of organ function. Since direct visualization of developing tissues, i.e., tissue sampling via sacrifice or biopsy, is invasive and wasteful, one challenge in TE/RM is establishing non-invasive tools to monitor the development of the constructs in vitro and in vivo. In addition, visualization of the transitional region between the tissue implant and the surrounding tissues is critical to the establishment of the overall success of the procedure. MRI is increasingly being used in TE/RM to monitor the remodeling and regeneration of engineered tissues. Bioengineers can now generate spatial maps of MR relaxation times, diffusion coefficients, magnetization transfer ratios, and the shear modulus to monitor, for example, new bone growth for prosthetic therapy in TE/RM. We hypothesize that MR can be extended to monitor quantitatively the growth of engineered tissues including bone, fat, and cartilage. By its periodic application in vitro and in vivo MR can enable the much anticipated successes in TE/RM. Our preliminary data demonstrate that quantitative MR methods can be applied to characterize non-invasively the changes associated with adipogenesis, osteogenesis, and chondrogenesis in tissue-engineered MSC-based constructs. This in vitro work established that MR acquired data can be directly correlated with the underlying tissue composition and structure as measured by biochemical and histological techniques. Our long term goal is to extend these methods to assess the structure and function of developing, engineered tissues in vivo. In order to achieve this goal we have assembled an interdisciplinary team of imaging scientists, tissue engineers and clinicians. The proposed work forms a 5-year plan to establish useful clinical MR tools for the optimization of TE/RM specifically for chondrogenic tissues. Public Health Relevance Statement (provided by applicant): The objective of this study is to develop new techniques for monitoring engineered chondrogenic tissues using magnetic resonance imaging and magnetic resonance elastography. Magnetic resonance imaging provides three dimensional views of developing tissue at all stages of growth without the need to sacrifice the animal or to biopsy the tissue. Magnetic resonance elastography gives a direct measure of the strength and stiffness of regenerating tissue: critical information needed to guide the design of new methods of tissue engineering and to assess the success of tissue implants for restoring tissue damaged by disease, injury or cancer treatment.

描述（由申请人提供）： 组织工程和再生医学（TE/RM）是一个不断发展的跨学科领域，它将工程学与生物学和医学相结合，用于功能组织和器官的发育。为了优化组织再生和器官功能恢复，植入前的体外监测和发育过程中的体内组织评估都至关重要。由于直接可视化发育​​中的组织，即通过牺牲或活检进行组织取样，是侵入性且浪费的，因此 TE/RM 的一项挑战是建立非侵入性工具来监测体外和体内构建体的发育。此外，组织植入物和周围组织之间过渡区域的可视化对于手术的整体成功至关重要。 MRI 越来越多地用于 TE/RM 来监测工程组织的重塑和再生。生物工程师现在可以生成 MR 弛豫时间、扩散系数、磁化传递比和剪切模量的空间图，以监测 TE/RM 中修复治疗的新骨生长等情况。我们假设 MR 可以扩展到定量监测工程组织（包括骨骼、脂肪和软骨）的生长。通过在体外和体内的定期应用，MR 可以在 TE/RM 领域取得令人期待的成功。我们的初步数据表明，定量 MR 方法可用于非侵入性地表征基于组织工程 MSC 的构建体中与脂肪生成、骨生成和软骨生成相关的变化。这项体外工作证实，MR 采集的数据可以与通过生化和组织学技术测量的底层组织成分和结构直接相关。我们的长期目标是扩展这些方法来评估体内发育的工程组织的结构和功能。为了实现这一目标，我们组建了一支由影像科学家、组织工程师和临床医生组成的跨学科团队。拟议的工作形成了一个五年计划，旨在建立有用的临床 MR 工具，以优化专门针对软骨形成组织的 TE/RM。 公共卫生相关性声明（由申请人提供）：本研究的目的是开发使用磁共振成像和磁共振弹性成像监测工程软骨形成组织的新技术。磁共振成像提供了发育各个阶段的发育组织的三维视图，无需处死动物或对组织进行活检。磁共振弹性成像可以直接测量再生组织的强度和刚度：指导组织工程新方法的设计以及评估组织植入物恢复因疾病、损伤或癌症治疗而受损的组织的成功所需的关键信息。