Identification of effectors of host cell killing by Entamoeba histolytica

溶组织内阿米巴杀伤宿主细胞效应子的鉴定

• 批准号: 7804335
• 负责人: Katherine S Ralston
• 金额: $ 1.54万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-17 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7804335
• 关键词: Acids; Amebiasis; Apoptosis; Apoptotic; Biological Assay; Bioterrorism; Blocking Antibodies; Caspase; Cause of Death; Cell membrane; Cells; Cessation of life; Child; Child health care; Cysteine Protease; Development; Disease; Drug Delivery Systems; Entamoeba histolytica; Goals; Human; Infection; Lactamase; Life; Light; Mass Spectrum Analysis; Morbidity - disease rate; Parasites; Pathogenesis; Plasmodium; Play; Proteins; Role; System; Testing; Vaccines; Vesicle; Video Microscopy; Virulence; Work; base; caspase-3; cell killing; cytotoxic; cytotoxicity; global health; killings; mortality; novel; novel therapeutics; overexpression; porin; protozoan amoebapore proteins; small hairpin RNA; trafficking

DESCRIPTION (provided by applicant): From this work, we expect to identify the effectors of cytotoxicity and to test if they need to be trafficked into host cells to exert their apoptotic effect. Successful completion of these studies will clarify the basic mechanisms of disease pathogenesis which will inform ongoing efforts in the development of an E. histolytica vaccine and new therapeutics and may shed light on strain-specific differences in amebic virulence. Since infection is common in children in the developing world and is a significant cause of morbidity and mortality, these studies have promise to positively impact global child health.

描述（由申请人提供）：通过这项工作，我们期望鉴定细胞毒性的效应物，并测试它们是否需要被运输到宿主细胞中以发挥其凋亡作用。这些研究的成功完成将阐明疾病发病机制的基本机制，这将为正在进行的溶组织内阿米巴疫苗和新疗法的开发工作提供信息，并可能揭示阿米巴毒力的菌株特异性差异。由于感染在发展中国家的儿童中很常见，并且是发病和死亡的重要原因，因此这些研究有望对全球儿童健康产生积极影响。