Mechanisms of Olfactory Transduction

嗅觉传导机制

• 批准号: 7850378
• 负责人: Barry W. ACHE
• 金额: $ 37.29万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-17 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7850378
• 关键词: Aging; Animal Model; Biochemical; Cations; Chemicals; Cloning; Competence; Complex; Cyclic Nucleotides; Disease; Event; Functional disorder; GTP-Binding Proteins; Inositol Phosphates; Invertebrates; Ion Channel; Lobster; Mediating; Molecular; Neurons; Nose; Odors; Olfactory Receptor Cells; Olfactory Receptor Neurons; Output; Pharmaceutical Preparations; Phosphatidylinositols; Phospholipids; Phosphorylation; Play; Quality of life; Receptor Cell; Regulation; Relative (related person); Role; Signal Pathway; Signal Transduction; Smell Perception; System; Taste Perception; in vivo; receptor

The sense of smell plays an increasingly appreciated role in our general quality of life and well being, a role that is compromised by the effect of disease, drugs, aging and environmental onslaught on our chemosensory competence. Receptor neurons in the nose serve the critical function of detecting and transducing odorants. Disruption of any of the intracellular events leading to receptor cell activation can result in olfactory dysfunction. Emerging evidence implicates phosphoinositide signaling and the transient receptor potential (TRP) ion channels commonly targeted by this signaling pathway in chemosensory transduction in taste receptor cells, vomeronasal receptor neurons, invertebrate olfactory receptor neurons, and possibly in vertebrate olfactory receptor neurons, where recent evidence suggests it can modulate the well established cyclic nucleotide signaling pathway. Phosphoinositide signaling is inherently complex, and this complexity has impeded a clear understanding of its role in many cellular systems. In this continuing project, we use an integrated, molecular, biochemical, and electrophysiologicalapproach to further define the cellular mechanisms of phosphoinositide signaling in an olfactory system. We use an animal model in which the functional role of phosphoinositide signaling in olfactory transduction is particularly well established, and has led to the evidence suggesting a potential role for phosphoinositide signaling in vertebrate olfaction. This project has the potential to identify new cellular and molecular events potentially involved in olfactory receptor cell activation, as well as contribute to a more general understanding of phospholipid signaling and TRP channel function in chemical sensing.

嗅觉在我们的整体生活质量和福祉中发挥着越来越重要的作用， 疾病、药物、衰老和环境对我们的影响会损害我们的作用 化学感应能力。鼻子中的受体神经元具有检测和识别的关键功能。 转换气味剂。任何导致受体细胞激活的细胞内事件的破坏都可以 导致嗅觉功能障碍。新出现的证据表明磷酸肌醇信号传导和短暂的 化学感应中该信号通路通常靶向的受体电位 (TRP) 离子通道 味觉受体细胞、犁鼻受体神经元、无脊椎动物嗅觉受体神经元的转导， 并且可能在脊椎动物的嗅觉受体神经元中，最近的证据表明它可以调节 完善的环核苷酸信号通路。磷酸肌醇信号传导本质上是复杂的，并且 这种复杂性阻碍了对其在许多蜂窝系统中的作用的清晰理解。在这持续的 项目中，我们使用综合的、分子的、生物化学的和电生理学的方法来进一步定义 嗅觉系统中磷酸肌醇信号传导的细胞机制。我们使用动物模型 磷酸肌醇信号在嗅觉转导中的功能作用特别好 已建立，并产生证据表明磷酸肌醇信号传导在 脊椎动物的嗅觉。该项目有可能识别新的细胞和分子事件 参与嗅觉受体细胞的激活，并有助于更普遍地理解 磷脂信号传导和 TRP 通道在化学传感中发挥作用。