Differential regulation of plant innate immunity

植物先天免疫的差异调节

基本信息

批准号：
7862794
负责人：
Ping He
金额：
$ 25.42万
依托单位：
TEXAS A&M UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-01 至 2015-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7862794
关键词：
Animals Arabidopsis Attenuated Binding Biochemical Biological Assay Biological Models Biological Process Calcium Cell surface Cells Communicable Diseases Complex Data Detection Employee Strikes Environment Environmental Risk Factor Event Family Family member Figs - dietary Gene Expression Profile Gene Expression Regulation Gene Family Genetic Genome Genomics Goals Immune Immune response Immune system Immunity Individual Infection Leucine-Rich Repeat Life Mammals Microbe Molecular Natural Immunity Nucleotides Pathogen detection Pattern Pattern recognition receptor Phosphotransferases Plant Model Plants Play Regulation Research Resources Role Signal Pathway Signal Transduction System Temperature Toll-like receptors Virulence Work base calcium-dependent protein kinase improved leucine-rich repeat protein member novel pathogen prevent public health relevance receptor sensor tool

项目摘要

DESCRIPTION (provided by applicant): Plants and animals rely on innate immunity to prevent potential infections by detection of pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRRs). In plants, PAMPs are perceived by cell-surface PRRs and mount PAMP- triggered immunity (PTI). Successful pathogens are able to suppress PTI by producing virulence effectors, which, in turn, are recognized by plant intracellular PRRs to initiate effector-triggered immunity (ETI). This complex host-microbe interaction is intricately intertwined with a wide range of environmental factors. For example, we found that the ambient temperature fluctuation regulates PTI and ETI in a distinct manner. We have developed an Arabidopsis cell system to express individual pathogen effectors for ETI or treat cells with purified PAMPs for PTI. In combination with enriched genetic and genomic resources in Arabidopsis, this synchronized and autonomous plant cell-single pathogen signal system holds significant promise for dissecting the complex regulation of signaling events in ETI and PTI. Our preliminary data strongly suggest the existence of differential early signaling mechanisms underlying two branches of plant innate immunity. In particular, we found that the specific members of a large gene family encoding calcium-dependent protein kinases (CDPKs) play pivotal roles in transducing calcium signature in ETI. Our proposed research will employ a multifaceted approach to further understand the signaling mechanisms underlying plant ETI and PTI. The Specific Aims for this application are the following: 1) Decipher the distinct and convergent gene regulation in ETI and PTI; 2) Elucidate the functions of CDPKs in ETI signaling; 3) Dissect the ambient temperature regulation of innate immunity. Plant PTI and ETI are most similar to "Toll-like receptor" and "NOD-like receptor" innate immune signaling pathways in mammals respectively. Thus, our research will contribute to a general understanding of innate immunity and improve our ability to prevent and control infectious diseases. PUBLIC HEALTH RELEVANCE: Understanding the intricately intertwined signal transduction networks acting downstream of various host immune sensors could provide novel avenues to prevent and control infectious diseases. Given that plant cell-surface and intracellular immune sensors are most similar to "Toll-like receptor" and "NOD-like receptor" in mammals respectively, our research will have broad impact on the understanding of signaling mechanisms of animal innate immunity.

描述（由申请人提供）：植物和动物依靠先天免疫通过模式识别受体（PRR）检测病原体相关分子模式（PAMP）来预防潜在的感染。在植物中，PAMP 被细胞表面 PRR 感知并引发 PAMP 触发免疫 (PTI)。成功的病原体能够通过产生毒力效应子来抑制 PTI，而毒力效应子又被植物细胞内 PRR 识别，从而启动效应子触发免疫 (ETI)。这种复杂的宿主-微生物相互作用与多种环境因素错综复杂地交织在一起。例如，我们发现环境温度波动以不同的方式调节 PTI 和 ETI。我们开发了拟南芥细胞系统来表达 ETI 的单个病原体效应子或用纯化的 PAMP 处理细胞以进行 PTI。结合拟南芥丰富的遗传和基因组资源，这种同步且自主的植物细胞-单一病原体信号系统对于剖析 ETI 和 PTI 信号事件的复杂调控具有重要前景。我们的初步数据强烈表明植物先天免疫的两个分支背后存在差异性早期信号机制。特别是，我们发现编码钙依赖性蛋白激酶 (CDPK) 的大基因家族的特定成员在 ETI 中的钙信号转导中发挥着关键作用。我们提出的研究将采用多方面的方法来进一步了解植物 ETI 和 PTI 的信号机制。本申请的具体目标如下： 1）破译ETI和PTI中不同和趋同的基因调控； 2) 阐明CDPKs在ETI信号传导中的功能； 3）剖析先天免疫的环境温度调节。植物PTI和ETI分别与哺乳动物的“Toll样受体”和“NOD样受体”先天免疫信号通路最相似。因此，我们的研究将有助于人们对先天免疫的普遍了解，并提高我们预防和控制传染病的能力。公共卫生相关性：了解作用于各种宿主免疫传感器下游的错综复杂的信号转导网络可以为预防和控制传染病提供新途径。鉴于植物细胞表面和细胞内免疫传感器分别与哺乳动物中的“Toll样受体”和“NOD样受体”最相似，我们的研究将对动物先天免疫信号机制的理解产生广泛的影响。