MEASUREMENT OF THE SPECTRAL SHIFT OF A NEW CLASS OF DNA BINDING DYES

新型 DNA 结合染料光谱位移的测量

• 批准号: 8169412
• 负责人: PAUL J SMITH
• 金额: $ 3.34万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169412
• 关键词: Binding; Cell Line; Cells; Collaborations; Computer Retrieval of Information on Scientific Projects Database; DNA Binding; Dyes; Fluorescence Spectroscopy; Funding; Grant; Image; In Situ; Institution; Life; Measurement; Performance; Research; Research Personnel; Resources; Sampling; Source; Staining method; Stains; United States National Institutes of Health; Universities; improved; instrument

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In collaboration with Dr. Paul Smith of Cardiff University, we will characterize the emission spectra of a new class of DNA binding dyes that are rapidly cell permeable and excite in the far red. The Cardiff group has characterized these dyes with a combination of conventional flow measurements, fluorescence spectroscopy of bulk samples and live cell spectral imaging. We will use stained cell lines to characterize the performance of the spectral instrument in the NIR region as compared to the other measurements. We will also use the improved PS cytometer to investigate changes in lifetime of these dyes upon DNA binding, potentially enabling separation of bound and unbound flurorophore in situ.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们将与卡迪夫大学的 Paul Smith 博士合作，表征一类新型 DNA 结合染料的发射光谱，这些染料可快速渗透细胞并激发远红光。 卡迪夫研究小组结合传统的流量测量、散装样品的荧光光谱和活细胞光谱成像来表征这些染料。 我们将使用染色的细胞系来表征光谱仪器在近红外区域与其他测量相比的性能。 我们还将使用改进的 PS 细胞仪来研究这些染料在 DNA 结合后寿命的变化，从而有可能实现原位结合和未结合荧光团的分离。