PROTEOMICS SHARED RESOURCE

蛋白质组学共享资源

• 批准号: 7944667
• 负责人: Scott A. Gerber
• 金额: $ 6.67万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-21 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944667
• 关键词: Acetylation; Address; Administrator; Alkylation; Amino Acid Sequence; Archives; Area; Arts; Back; Bar Codes; Bioinformatics Shared Resource; Biology; Bite; Businesses; Cancer Center; Cancer Center Support Grant; Charge; Client; Code; Commit; Communities; Complex Mixtures; Computer Assisted; Computer software; Computers; Consult; Consultations; Core Facility; Cost Analysis; Cost Savings; DNA; Data; Data Analyses; Data Files; Data Reporting; Databases; Digestion; Drops; Electronic Mail; Electronics; Electrophoresis; Ensure; Equipment; Experimental Designs; Faculty; Feedback; Foundations; Funding; Future; Gel; Grant; Growth; High Pressure Liquid Chromatography; Hour; Housing; Immunoprecipitation; Individual; Institution; Ions; Laboratories; Laboratory Research; Maintenance; Manuals; Mass Spectrum Analysis; Methods; Methylation; Mission; Modification; Molecular Biology; Molecular Weight; Names; Norris Cotton Cancer Center; Operative Surgical Procedures; Peptide Sequence Determination; Peptides; Phase Transition; Phosphorylation; Post Translational Modification Analysis; Post-Translational Protein Processing; Price; Process; Protein Analysis; Proteins; Proteolysis; Proteomics; Proteomics Shared Resource; Protocols documentation; Pump; Recruitment Activity; Reporting; Research; Research Personnel; Resource Sharing; Resources; Running; Sampling; Security; Services; Silver; Silver Staining; Site; Solvents; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staging; Staging System; Staining method; Stains; Structure; System; Technology; Time; Training; Trypsin; Update; Validation; Writing; base; chemical synthesis; cluster computing; computing resources; cost; data acquisition; data integrity; design; detector; encryption; falls; firewall; functional group; instrument; instrumentation; interest; liquid chromatography mass spectrometry; mass spectrometer; medical schools; meetings; member; parallel processing; protein complex; research study; skills; small molecule; trafficking; web site

The mission of the Proteomics Shared Resource (PRSR) at the Norris Cotton Cancer Center (NCCC) is to provide NCCC investigators with local access to state-of-the-art proteomic technologies, scientific consulting For experimental design, consultation regarding results interpretation, and ancillary bioanalytical support services at affordable rates. Newly established as an independent NCCC Shared Resource, the PRSR in Fall 2007 completed its transition from providing limited proteomic services as part of the former Molecular Biology and Proteomics Shared Resource to full-fledged independent operation as a stand-alone core facility. During this transition, the PRSR acquired newly renovated laboratory space in the NCCC Rubin Building research labs, modernized its mass spectrometry and liquid chromatography instrumentation and expanded its computational resources, appointed proteomics faculty recruit Dr. Scott A. Gerber as Director of the PRSR, and expanded its service portfolio to include protein post-translational modification analysis and complex protein mixture analysis. These efforts collectively represent a $2.8 million commitment from the NCCC and From Dartmouth Medical School to bring a modern proteomics platform within reach of Cancer Center investigators and the greater Dartmouth research community as a whole. During this one-year transition phase, the PRSR received $12,700 in chargebacks from 9 NCCC research laboratories, or 87% of the total ($14,600) chargebacks for the facility (from a collective 16 Dartmouth laboratories). We estimate that the total operating cost of the PRSR for FY 2008 (year 1 of the CCSG renewal) will be $326,760 and anticipate $40,000 in total chargebacks, requiring $48,349 from CCSG and $238,411 from other sources for NCCC investigators (75% of the shortfall). Owing to the rapid increase in demand for PRSR services over the past year, we anticipate strong growth in the rate of chargebacks for the PRSR such that the large subsidy by NCCC and the institution can be reduced. Currently, support funds for the PRSR in transition are provided by multiple institutional sources.

诺里斯·科顿癌症中心 (NCCC) 蛋白质组学共享资源 (PRSR) 的使命是 为 NCCC 研究人员提供本地最先进的蛋白质组技术和科学咨询 用于实验设计、有关结果解释的咨询以及辅助生物分析支持 以实惠的价格提供服务。 PRSR 作为一个独立的 NCCC 共享资源新成立，于 2007 年秋季完成了其 从作为前分子生物学和蛋白质组学的一部分提供有限的蛋白质组学服务转变 共享资源到作为独立核心设施的全面独立运营。在这个转变过程中， PRSR 在 NCCC Rubin Building 研究实验室购买了新装修的实验室空间， 对其质谱和液相色谱仪器进行了现代化改造，并扩大了其 计算资源，任命蛋白质组学教授 Scott A. Gerber 博士为 PRSR 主任， 并扩展了其服务组合，包括蛋白质翻译后修饰分析和复杂的分析 蛋白质混合物分析。这些努力总共代表了 NCCC 和 达特茅斯医学院将现代蛋白质组学平台带入癌症中心 研究人员和整个达特茅斯研究界。 在这一一年的过渡阶段，PRSR 从 9 项 NCCC 研究中收到了 12,700 美元的退款 实验室，或该设施退款总额（14,600 美元）的 87%（来自达特茅斯学院的 16 个集体） 实验室）。我们估计 2008 财年（CCSG 第 1 年）PRSR 的总运营成本 续订）费用为 326,760 美元，预计退款总额为 40,000 美元，需要 CCSG 支付 48,349 美元， 来自其他来源的 238,411 美元用于 NCCC 调查人员（缺口的 75%）。由于快速增加 由于过去一年对 PRSR 服务的需求，我们预计退款率将强劲增长 PRSR使得NCCC和机构的大量补贴可以减少。目前，扶持资金 转型中的 PRSR 由多个机构来源提供。