Mouse and cMyBP-C Protein Production Core

小鼠和 cMyBP-C 蛋白质生产核心

• 批准号: 7789884
• 负责人: Jeffrey Robbins
• 金额: $ 30.53万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2015-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7789884
• 关键词: Antibodies; Biology; Cardiovascular system; Equipment and supply inventories; Foundations; Housing; In Vitro; Individual; Maintenance; Medical center; Molecular; Mouse Protein; Mus; Pediatric Hospitals; Preparation; Production; Program Research Project Grants; Protein Fragment; Proteins; Quality Control; Research Personnel; Scientist; Shipping; Ships; System; Transgenic Mice; base; citrate carrier; design; member; myosin-binding protein C

This Core will be housed at the Cincinnati Children's Hospital Medical Center within the Division of Molecular Cardiovascular Biology. Although spatially separated from members of the Program Project Grant team. Dr. Robbins has been collaborating with them for years and supplies the current Program Project Grant investigators and Cores with both mice and protein fragments. The Core will be critical for the studies proposed in all of the Program Project Grant's Component's as it will be responsible for the design, production, colony maintenance and shipment of ail transgenic mice that are proposed by the Program Project Grant's investigators and Core B. The Core will also produce the different proteins, protein fragments and antibodies needed for the in vitro and isolated systems studies. As such, it forms an integral part of the Program Project Grant's foundation. The Core's responsibilities are: ¿ Design and build all constructs for bacterial- or Sacu/o-based MyBP-C protein and protein fragment production. ¿ Grow and purify all protein fragments in sufficient quantities to satisfy the investigators' needs ¿ Design, build and generate all transgenic mice that are proposed in the Program Project Grant. ¿ Maintain sufficient numbers of all lines in barrier facilities so that they can be distributed as needed to the investigators as well as to other scientists who request them. ¿ Serve as a central clearing house for the preparation, maintenance and quality control of the cMyBP-C specific antibodies ¿ Maintain an adequate inventory of all of the above and arrange for convenient shipping to the individual investigators or Core Leaders.

该核心将安置在辛辛那提儿童医院医疗中心分子科内 心血管生物学虽然与项目资助团队的成员在空间上分开。 罗宾斯已与他们合作多年，并提供当前的计划项目拨款 研究人员和核心人员与小鼠和蛋白质片段。 核心对于所有计划项目补助金的组成部分中提出的研究至关重要，因为它将 负责所有转基因小鼠的设计、生产、群体维护和运输 由计划项目拨款的研究人员和核心 B 提出。核心还将产生不同的 体外和分离系统研究所需的蛋白质、蛋白质片段和抗体。 构成计划项目拨款基金会的一个组成部分。 核心的职责是： ¿设计和构建用于细菌或基于 Sacu/o 的 MyBP-C 蛋白和蛋白片段生产的所有构建体。 ¿生长并纯化足够数量的所有蛋白质片段，以满足研究人员的需求 ¿设计、构建和生成计划项目拨款中提出的所有转基因小鼠。 ¿在屏障设施中保留足够数量的所有管线，以便可以根据需要将其分发给研究人员以及需要它们的其他科学家。 ¿充当 cMyBP-C 特异性抗体的制备、维护和质量控制的中央信息交换所 ¿保持上述所有物品的充足库存，并安排方便地运送给个别调查员或核心领导者。