F. nucleatum in periodontal disease and preterm birth

具核梭杆菌在牙周病和早产中的作用

• 批准号: 7897931
• 负责人: Yiping Han
• 金额: $ 11.83万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7897931
• 关键词: Abscess; Address; Adherence; Affinity; Amniotic Fluid; Animal Model; Antibodies; Applications Grants; Award; Bacteria; Bacterial Adhesins; Basic Science; Binding; Biological Assay; Blood; Body part; Cell Communication; Cell-Matrix Junction; Characteristics; Chromatography; Clinical Research; DNA Fingerprinting; Dental Plaque; Dentistry; Doctor of Philosophy; Education; Educational process of instructing; Endothelial Cells; Epithelial; Faculty; Fetal Membranes; Funding; Fusobacteria; Fusobacterium; Fusobacterium nucleatum; Future; Goals; Gram-Negative Anaerobic Bacteria; Health; Hematogenous; Heterogeneity; Human; Immune response; In Vitro; Independent Scientist Award; Infection; Inflammatory; Injection of therapeutic agent; Kinetics; Laboratories; Left; Location; Low Birth Weight Infant; Maternal Health; Mediating; Modeling; Mus; NIH Program Announcements; National Institute of Dental and Craniofacial Research; Oral; Oral cavity; Oral health; Organism; Periodontal Diseases; Pilot Projects; Placenta; Polymerase Chain Reaction; Precipitation; Pregnant Women; Premature Birth; Premature Labor; Proliferating; Reproductive Biology; Research; Research Support; Risk Factors; Role; Sample Size; Sampling; Source; Structure; Testing; Tissues; Toll-like receptors; Translational Research; Universities; Uterus; Vagina; Virulence; Woman; career; crosslink; cytokine; frontier; in vivo; interest; mutant; novel; oral tissue; pregnant; programs; receptor; stillbirth

DESCRIPTION (provided by applicant): Dr Yiping Han is a PhD microbiologist. Dr Han's research evolves around understanding the virulence mechanism of F. nucleatum. Since joining the faculty at Case Western Reserve University, her research interest has expanded from basic science to include more clinically oriented studies. Dr Han and her colleagues recently developed a pregnant murine model and showed a causal relationship between F. nucleatum and preterm birth. Dr Han is using F. nucleatum as a model organism to investigate the correlation between oral health and preterm birth. She has research support from NIDCR, with an R03 award (DE 14447) to investigate the source of F. nucleatum infection in amniotic fluids in women in preterm labor, and an R01 award (DE 14924) to further characterize the FadA adhesin from this organism. Support from a K02 award will allow Dr Han to forego further grant applications for the near future and to avoid projected increase in teaching and administrative responsibilities. This will permit Dr Han to devote over 80% of her effort on her research program and her plans for re-education in dentistry and reproductive biology. Long term career goals are to become a well-rounded oral microbiologist competent in both basic science and translational research. The specific aims of this application are: (i) to characterize the FadA adhesin from F. nucleatum for its role in tissue cell attachment and invasion; (ii) to identify the host receptor for FadA and to characterize its role in F. nudeatum-hosl cell interactions; and (iii) to determine if women with amniotic fluid infections of F. nucleatum harbor the same species in the vaginal track, blood, or the oral cavity.

描述（由申请人提供）：韩一平博士是微生物学家博士。韩博士的研究围绕着了解具核梭杆菌的毒力机制展开。自从加入凯斯西储大学任教以来，她的研究兴趣已从基础科学扩展到更多以临床为导向的研究。韩博士和她的同事最近开发了一种怀孕小鼠模型，并显示了具核梭菌与早产之间的因果关系。韩博士正在使用具核梭杆菌作为模式生物来研究口腔健康与早产之间的相关性。她获得了 NIDCR 的研究支持，并获得 R03 奖 (DE 14447) 来研究早产妇女羊水中具核梭菌感染的来源，并获得 R01 奖 (DE 14924) 来进一步表征该生物体的 FadA 粘附素。 K02 奖项的支持将使韩博士能够在不久的将来放弃进一步的资助申请，并避免教学和行政职责的预计增加。这将使韩博士能够将超过 80% 的精力投入到她的研究项目以及牙科和生殖生物学再教育计划上。长期职业目标是成为一名能够胜任基础科学和转化研究的全面口腔微生物学家。本申请的具体目的是：(i) 表征具核梭杆菌的 FadA 粘附素在组织细胞附着和侵袭中的作用； (ii) 鉴定 FadA 的宿主受体并表征其在 F. nuvatum-hosl 细胞相互作用中的作用； (iii) 确定羊水感染具核梭杆菌的女性在阴道、血液或口腔中是否存在相同的物种。

项目成果

Signal peptide of FadA adhesin from Fusobacterium nucleatum plays a novel structural role by modulating the filament's length and width.

来自具核梭杆菌的 FadA 粘附素信号肽通过调节丝的长度和宽度发挥新的结构作用。

• 发表时间: 2012-01-02
• 影响因子: 3.5
• 作者: Témoin, Stéphanie;Wu, Karen L;Wu, Vivian;Shoham, Menachem;Han, Yiping W
• 通讯作者: Han, Yiping W

Transmission of diverse oral bacteria to murine placenta: evidence for the oral microbiome as a potential source of intrauterine infection.

多种口腔细菌向小鼠胎盘的传播：口腔微生物组作为宫内感染潜在来源的证据。

• 发表时间: 2010-04
• 影响因子: 3.1
• 作者: Fardini, Yann;Chung, Peter;Dumm, Rochelle;Joshi, Nishiant;Han, Yiping W
• 通讯作者: Han, Yiping W

IFPA Meeting 2010 Workshop Report I: Immunology; ion transport; epigenetics; vascular reactivity; epitheliochorial placentation; proteomics.

IFPA 2010 年会议研讨会报告 I：免疫学；

• 发表时间: 2011-03
• 影响因子: 3.8
• 作者: Abad, C;Antczak, D F;Carvalho, J;Chamley, L W;Chen, Q;Daher, S;Damiano, A E;Dantzer, V;Díaz, P;Dunk, C E;Daly, E;Escudero, C;Falcón, B;Guillomot, M;Han, Y W;Harris, L K;Huidobro;Illsley, N;Jammes, H;Jansson, T;Johnson, G A;K
• 通讯作者: K

Acute chorioamnionitis at term caused by the oral pathogen Fusobacterium nucleatum.

由口腔病原体具核梭杆菌引起的足月急性绒毛膜羊膜炎。

• 发表时间: 2012-10-01
• 期刊: Hawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health
• 作者: J. Bohrer;L. Kamemoto;Pamela G Almeida;K. Ogasawara
• 通讯作者: K. Ogasawara

Can oral bacteria cause pregnancy complications?

口腔细菌会导致妊娠并发症吗？

• 发表时间: 2011-07
• 作者: Han; Yiping Weng
• 通讯作者: Yiping Weng