Multi-photon laser scanning microscope

多光子激光扫描显微镜

• 批准号: 7498307
• 负责人: FRANCISCO J ALVAREZ
• 金额: $ 71.81万
• 依托单位: WRIGHT STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7498307
• 关键词: Adult; Arts; Brain Stem; Cell Volumes; Collaborations; Core Facility; Dendrites; Development; Disease; Electron Microscope; Funding; Generations; Goals; Grant; Health; Housing; Hypercapnic respiratory failure; Image; Image Analysis; Injury; Ion Channel; Lasers; Membrane; Microscope; Minor; Molecular; Motor; Nervous System Physiology; Neuroglia; Neurons; Neurosciences; Neurosciences Research; Recovery; Recovery of Function; Renshaw Cell; Research; Research Personnel; Role; Scanning; Signal Transduction; Sleep Apnea Syndromes; Spinal Cord; Spinal cord injury; Sudden infant death syndrome; Surface; Syndrome; Technical Expertise; United States National Institutes of Health; Universities; base; instrument; multi-photon; nerve injury; public health relevance; respiratory; transmission process; two-photon

DESCRIPTION (provided by applicant): The goal of this proposal is to purchase/acquire and implement a Two-photon microscope which will primarily be utilized to strengthen the neuroscience research at Wright State, and permit key investigators to perform studies they would otherwise be unable to accomplish. We have assembled a team of highly accomplished researchers who will constitute the major users of the requested instrument and all of which are PIs on NIH funded grants (R01s). Dr. Francisco Alvarez will use the requested instrument to study Generation and functional role of Renshaw cells in the developing spinal cord. This study has broad implications on motor development and reconfiguration and recovery of function of neurons in adults after spinal cord injuries that disrupt these intraspinal circuits. Dr. Francisco Alvarez-Leefman will utilize the microscope to understand the cellular and molecular mechanisms underlying cell volume control in neurons and glial cells. Dr. Robert Fyffe will utilize the microscope to investigate the dynamics of surface membrane ion channel localization following altered activity or axonal injury, a project that also has implications in the field of spinal cord/nerve injury and recovery. Dr. Robert Putnam will use the requested instrument to study the role of Intracellular Ca and pH in chemosensitive signaling in dendrites of brainstem neurons. A greater understanding of central chemosensitive signaling will open new targets for treatments aimed at altering respiratory drive in such disorders as central hypoventilation syndrome, SIDS, and sleep apnea. The instrument will be housed and fully supported in a core facility in the recently created Comprehensive Neuroscience Center, where, in addition to these four major users, there will be opportunity for the instrument to be used by additional staff/Minor users/ Junior Investigators for cellular and molecular studies. Based on our current expertise as well as our collaboration with Dr Michael O'Donovan at NIH (who has invited Dr. Alvarez for a month to use a two-photon microscope at NIH) we believe that we will master the use of the Two-photon microscope and will be able to make the most of its potential. A Two-photon microscope will enhance the Imaging Core Facility at Wright State which currently houses two confocal microscopes and a transmission electron microscope, all networked to off-line image analysis workstations. The technical expertise to be gained will be utilized to further advance other research endeavors at WSU. PUBLIC HEALTH RELEVANCE: A Two-Photon Microscope will enhance the Neuroscience core at Wright State University and promote state- of-the art scientific research. The research proposed here will make use of this instrument to its full capacity, leading to an understanding of both neurophysiologic and neuropathologic mechanisms of high relevance to nervous system function in health and disease or injury conditions.

描述（由申请人提供）：该提案的目标是购买/获得并实施双光子显微镜，该显微镜将主要用于加强赖特州立大学的神经科学研究，并允许主要研究人员进行他们原本无法进行的研究来完成。我们组建了一支由高度成就的研究人员组成的团队，他们将构成所请求仪器的主要用户，并且所有这些人都是 NIH 资助的赠款 (R01s) 的 PI。 Francisco Alvarez 博士将使用所需的仪器来研究 Renshaw 细胞在发育中的脊髓中的生成和功能作用。这项研究对成人脊髓损伤后脊髓内回路破坏后神经元的运动发育、重新配置和功能恢复具有广泛的影响。 Francisco Alvarez-Leefman 博士将利用显微镜来了解神经元和神经胶质细胞中细胞体积控制的细胞和分子机制。 Robert Fyffe 博士将利用显微镜研究活动改变或轴突损伤后表面膜离子通道定位的动态，该项目对脊髓/神经损伤和恢复领域也有影响。 Robert Putnam 博士将使用所需的仪器来研究细胞内 Ca 和 pH 在脑干神经元树突化学敏感信号传导中的作用。对中枢化学敏感信号传导的更深入了解将为旨在改变中枢通气不足综合征、SIDS 和睡眠呼吸暂停等疾病的呼吸驱动的治疗开辟新的目标。该仪器将安置在最近创建的综合神经科学中心的核心设施中并得到充分支持，除了这四个主要用户之外，该仪器还将有机会被其他工作人员/次要用户/初级研究人员使用细胞和分子研究。基于我们目前的专业知识以及我们与 NIH 的 Michael O'Donovan 博士的合作（他邀请 Alvarez 博士在 NIH 使用双光子显微镜一个月），我们相信我们将掌握双光子显微镜的使用光子显微镜并将能够充分发挥其潜力。双光子显微镜将增强莱特州立大学的成像核心设施，该设施目前拥有两台共焦显微镜和一台透射电子显微镜，所有这些都与离线图像分析工作站联网。获得的技术专业知识将用于进一步推进华盛顿州立大学的其他研究工作。公共健康相关性：双光子显微镜将增强莱特州立大学的神经科学核心，并促进最先进的科学研究。这里提出的研究将充分利用该仪器，从而了解与健康和疾病或损伤条件下的神经系统功能高度相关的神经生理学和神经病理学机制。