Core--Laboratory

核心--实验室

• 批准号: 7979781
• 负责人: DAVID H. MARTIN
• 金额: $ 13.34万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7979781
• 关键词: Aerobic; Anaerobic Bacteria; Antibiotic susceptibility; Area; Bacteria; Biological Assay; Cell Culture Techniques; Cell Line; Chlamydia trachomatis; Clinical Research; Complex; Computers; Cytokeratin; Data; Databases; Development; Disease; Ecosystem; Endocervix; Epithelial Cells; Exocervix; Genotype; Gonorrhea; Health; Individual; Institution; Laboratories; Laboratory Research; Local Microbicides; Location; Methods; Molecular; Mycoplasma; Mycoplasma genitalium; Nucleic Acid Amplification Tests; Nucleic Acids; Organism; Research; Research Personnel; Resources; Role; Shipping; Ships; Site; Specimen; Specimen Handling; Swab; System; Techniques; Technology; Testing; Time; Trichomonas vaginalis; Vagina; computerized; denaturing gradient gel electrophoresis; immortalized cell; improved; meetings; tool

Core B: This laboratory core will provide high quality laboratory technology in support of all four projects proposed in this STI TM CRC application including: organism culture for Chlamydia trachomatis, Trichomonas vaginalis, aerobic and anaerobic bacteria and mycoplasmas; antibiotic susceptibility testing of bacteria and T. vaginalis; real time PCR quantification of T. vaginalis; genotyping of C. trachomatis; nucleic acid amplification assys for C. trachomatis, Neiserria gonorrhoeae and Mycoplasma genitalium; and epithelial cell cultures. Additionally the laboratory core will provide a central specimen processing and distribution resource that will coordinate distribution of specimens to the core laboratory and to the individual research laboratories and will coordinate shipping and receiving specimens to and from collaborating institutions. Computer data bases will be used to track specimen distribution and storage locations of remnant specimens, cultured organisms and PCR products. Finally the lab core will further development of denaturing gradient gel electrophoresis technology as a tool for the study of the vaginal ecosystem including analysis of the explanatory values of the approach, improving assay throughput, development of computerized systems to manage the complex data produced by this method and application of the technique to sequential vaginal swab specimens that will be obtained in the course of studies that will be carried out in several of the CRC projects. All of these activites are essential for meeting the objectives of the Gulf South STI TM CRC as stated in the Overview section of this proposal.

核心B：该实验室核心将提供高质量的实验室技术来支持所有四个项目 本 STI TM CRC 申请中提出的内容包括：沙眼衣原体生物培养， 阴道毛滴虫、需氧和厌氧细菌以及支原体；抗生素敏感性试验 细菌和阴道毛滴虫；阴道毛滴虫的实时 PCR 定量；沙眼衣原体的基因分型；核酸 用于沙眼衣原体、淋病奈瑟菌和生殖支原体的酸扩增分析系统；和 上皮细胞培养物。此外，实验室核心将提供中央样本处理和 分配资源，将协调样本向核心实验室和个人的分配 研究实验室并将协调合作的标本运输和接收 机构。计算机数据库将用于跟踪样本分布和存储位置 残留标本、培养生物和 PCR 产物。最后实验室核心将进一步发展 变性梯度凝胶电泳技术作为研究阴道生态系统的工具，包括 分析该方法的解释值，提高测定通量，开发 计算机化系统来管理由该方法和应用程序产生的复杂数据 技术连续阴道拭子标本将在研究过程中获得 在几个 CRC 项目中实施。 所有这些活动对于实现海湾南部 STI TM CRC 的目标至关重要，如 本提案的概述部分。