Prenatal Cigarette Smoke Exposure: Impact on Offspring Gut Bacterial Microbiome

产前香烟烟雾暴露：对后代肠道细菌微生物组的影响

基本信息

批准号：
10747173
负责人：
CYNTHIA CORBITT
金额：
$ 14.84万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-03-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10747173
关键词：
Acetates Age Age Months Air Attenuated Award Behavior Disorders Behavioral Biological Assay Biomedical Research Birth Weight Birth length Blood Glucose Brain Calorimetry Carbohydrates Cecum Characteristics Chemicals Child Coupled Data Development Dual-Energy X-Ray Absorptiometry Dyslipidemias Eating Eligibility Determination Euthanasia Exhibits Family Fasting Feces Female Fermentation Firmicutes First Generation College Students Funding Gene Expression Genes Glucose Glucose Intolerance Glucose tolerance test Goals Grant Growth Health Hepatic High Fat Diet Hormones Hour Immune system Immunologist Inflammation Inhalation Insulin Intervention Intestines Kentucky Latino Link Lipids Liver Low Birth Weight Infant Lupus Measures Mediating Metabolic Metabolic syndrome Modeling Mus Nutritional Organ Overweight Pancreas Parents Phenotype Plasma Pregnancy Production Proteobacteria Protocols documentation Puberty Publications Reporting Research Research Training Resistance Rural Same-sex Scanning Science Serum Signal Transduction Smoke Starch Structure Students Supplementation Testing Transplantation Underrepresented Minority Variant Volatile Fatty Acids Weaning Woman Work Writing absorption bacterial community bacteriome beta diversity career cigarette smoking dietary disadvantaged background dysbiosis economic disparity environmental tobacco smoke exposure exposure to cigarette smoke fetal tobacco exposure functional disability glucagon-like peptide 1 graduate school graduate student gut dysbiosis gut microbiome gut microbiota impaired glucose tolerance in utero insulin sensitivity interest male metabolic phenotype metabolomics microbial microbial composition microbiome microbiome alteration microbiome composition microbiota mouse model obesity risk offspring outcome disparities postnatal pregnant prenatal pup sex smoking during pregnancy symposium transcriptome sequencing transcriptomics

项目摘要

ABSTRACT This Research Supplement to Promote Diversity in Health-Related Research is to support a graduate student to perform research funded by R15 award 2R15ES028440-02A1, Prenatal Cigarette Smoke Exposure: Impact on Offspring Gut Bacterial Microbiome. Mr. Isaiah Burciaga is eligible for this award on multiple fronts: he is an underrepresented minoritized (Afro-Latino) student in science from a rural, economically disadvantaged background who is a first-generation college student. He has demonstrated interest in a biomedical research career and has exhibited ability both academically and technically to fulfill that career goal. The studies proposed within the parent award use a well-developed murine model of developmental exposure to tobacco smoke. We have shown effects of prenatal cigarette smoke exposure (CSE) on birth weight, catch-up growth, and gut bacterial microbiome in mouse dams and their offspring, as well as effects on offspring phenotype. However, we do not know if the observed changes in microbiome and offspring phenotype are linked. We will measure the effect of cecal transfer of microbiomes or dietary resistant starch on those same measures in this model. Our central hypothesis, that CSE-induced gut microbiome alterations are linked to offspring phenotype, will be tested within 2 aims: Aim 1: use cecal transfer in weanlings to separate effects of CSE-induced microbiome from CSE itself Aim 2: use dietary resistant starch to increase short-chain fatty acids before vs after puberty If our central hypothesis is supported, additional studies will delve into gut-liver-brain axis consequences of intestinal dysbiosis, as well as investigate potential interventions. Mr. Burciaga’s research training will focus primarily on Aim 2 and will include participation in professional development activities through the UofL Graduate School, participation in scientific conferences, grant writing, and publication.

抽象的本研究补充促进健康相关研究的多样性，旨在支持研究生开展由 R15 奖项 2R15ES028440-02A1 资助的研究，产前香烟烟雾暴露：影响 Isaiah Burciaga 先生在多个方面都有资格获得此奖项：他是一位来自经济弱势的农村地区的少数族裔（非裔拉丁裔）科学学生他是第一代大学生，对生物医学研究表现出兴趣。职业生涯，并在学术和技术上表现出实现该职业目标的能力。家长奖中提议使用成熟的烟草发育暴露小鼠模型我们已经证明了产前香烟烟雾暴露 (CSE) 对出生体重、追赶性生长、小鼠及其后代的肠道细菌微生物组，以及对后代表型的影响。然而，我们不知道观察到的微生物组变化和后代表型是否相关。测量盲肠转移微生物组或膳食抗性淀粉对本文中相同措施的影响我们的中心假设是，CSE 引起的肠道微生物组改变与后代表型有关，将在 2 个目标内进行测试：目标 1：在断奶仔猪中使用盲肠移植来分离 CSE 诱导的微生物组与 CSE 本身的影响目标 2：在青春期前后使用膳食抗性淀粉增加短链脂肪酸如果我们的中心假设得到支持，更多的研究将深入探讨肠-肝-脑轴的后果 Burciaga 先生的研究培训将重点关注肠道菌群失调以及调查潜在的干预措施。主要针对目标 2，并将包括通过伦敦大学毕业生参与专业发展活动学校、参加科学会议、资助写作和出版。