Washington University Chronic KidneyDisease National Resource Center

华盛顿大学慢性肾病国家资源中心

基本信息

批准号：
10747719
负责人：
BENJAMIN D. HUMPHREYS
金额：
$ 90.48万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10747719
关键词：
Acceleration Address Adopted Affect Area Bar Codes Benign Biological Assay CRISPR/Cas technology Cardiovascular system Cell Culture Techniques Cell Nucleus Cells Chronic Chronic Kidney Failure Code Communication Communities Consultations Data Analyses Development Disease Diverse Workforce Doctor of Medicine Doctor of Philosophy Education Equipment Evolution Family Fibrosis Functional disorder Funding Genes Genetic Genetic Techniques Genomic medicine Genus Hippocampus Goals In Vitro Incubators Injury Injury to Kidney Kidney Lead Leadership Metabolic Metabolism Modeling Morbidity - disease rate Mus Pathogenicity Pathologic Patients Population Pre-Clinical Model Proteins Protocols documentation RNA Splicing Radioactive Research Research Personnel Resolution Resource Development Resource Sharing Resources Scientific Advances and Accomplishments Services Students Techniques Technology Testing Time Tissues Tracer Training Tubular formation Underrepresented Minority United States National Institutes of Health Universities Validation Variant Washington Work bioinformatics pipeline biomedical resource computational pipelines cost genetic testing genetic variant hands on instruction improved in silico in situ sequencing in vitro Assay in vivo kidney biopsy kidney metabolism metabolomics mortality multiple omics novel therapeutic intervention oxidation programs recruit response to injury single cell technology stable isotope summer student technological innovation tool transcriptomics variant of unknown significance

项目摘要

Project Summary/Abstract: Overall Component The Washington University Chronic Kidney Disease National Resource Center is focused on the significant problem of chronic kidney disease (CKD), which affects almost 15% of the US population and carries significant morbidity and mortality. Several scientific advances have the promise to accelerate CKD research, but many of these advances are not accessible to the kidney research community due to limited expertise and/or the need for expensive equipment. Thus, this NRC will address the fundamental challenge of providing better access to cutting-edge techniques in single cell omics, genetics, and metabolism to the kidney research community to facilitate advances in CKD research. Dr. Ben Humphreys is the overall program director, and this Center will consist of four Cores: an Administrative Core, two Biomedical Resource Cores, and a Resource Development Core. The Administrative Core will oversee distribution of funds across the Cores, communicate with the National O’Brien Consortium, manage the Summer Student Enrichment Program, and promote the involvement of early stage investigators and a diverse workforce. The Variant Validation Core, one of the Biomedical Resource Cores, investigates the pathogenicity of genetic variants of uncertain significance using CRISPR/Cas9-gene editing, in silico approaches, and tailored in vitro assays. The Metabolism Core, the other Biomedical Resource Core, provides consultation for users and access to a number of metabolic assays to interrogate changes in kidney metabolism relevant to CKD. These assays include Seahorse bioflux analysis, radioactive substrate oxidation assays of tissue ex vivo, untargeted metabolomics, and stable isotope tracer studies. The Metabolism Core will also work with the O’Brien Consortium to provide validated protocols for assays commonly performed (e.g. Seahorse assays on primary cells) and provide hands’ on training using these protocols. The Single Cell Omics Research Evolution (SCORE) Core is the Resource Development Core, which develops protocols and bioinformatics pipelines for cutting-edge techniques like split pool barcoding for single nuclei multi-omics and high resolution in-situ sequencing-based spatially resolved transcriptomics. All four Cores will work together and with the network of O’Brien National Resource Centers to make scientific advancements more accessible to the kidney research community with particular emphasis on junior investigators and development of a diverse biomedical workforce.

项目摘要/摘要：总体组成部分华盛顿大学慢性肾病国家资源中心专注于重要的慢性肾脏病 (CKD) 问题影响着近 15% 的美国人口，并携带显着的发病率和死亡率多项科学进展有望加速 CKD 研究，但由于专业知识有限，肾脏研究界无法获得其中许多进展和/或对昂贵设备的需求因此，该 NRC 将解决提供的根本挑战。更好地获得单细胞组学、遗传学和肾脏研究代谢方面的尖端技术本·汉弗莱斯 (Ben Humphreys) 博士是该项目的总体主任，旨在促进 CKD 研究的进展。中心将由四个核心组成：一个行政核心、两个生物医学资源核心和一个资源核心开发核心。行政核心将监督核心之间的资金分配，进行沟通。与国家奥布莱恩联盟合作，管理暑期学生强化计划，并促进早期研究人员和多元化工作人员的参与，是变体验证核心之一。生物医学资源核心，利用技术研究不确定意义的遗传变异的致病性另一种是 CRISPR/Cas9 基因编辑、计算机方法和定制的体外测定。生物医学资源核心，为用户提供咨询并访问多种代谢测定，以询问与 CKD 相关的肾脏代谢变化。这些检测包括 Seahorse 生物通量分析、离体组织的放射性底物氧化测定、非靶向代谢组学和稳定同位素示踪剂代谢核心还将与奥布莱恩联盟合作，为以下研究提供经过验证的方案。常用的检测（例如原代细胞的海马检测）并提供使用培训这些协议是单细胞组学研究进化 (SCORE) 的核心。 Core，为 split pool 等尖端技术开发协议和生物信息学管道基于空间分辨的单核多组学条形码和高分辨率原位测序所有四个核心将与奥布莱恩国家资源中心网络一起工作。使肾脏研究界更容易获得科学进步，特别强调初级研究人员和多元化生物医学队伍的发展。