Model Systems Core

模型系统核心

基本信息

批准号：
10747626
负责人：
AROHAN R SUBRAMANYA
金额：
$ 29.59万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10747626
关键词：
Acceleration Acids Animal Model Animals Applications Grants Bioenergetics Biological Models Blinded CRISPR/Cas technology Cardiovascular system Catalogs Cell Culture Techniques Cell Line Cell Separation Cell Volumes Cells Collaborations Communities Complex Computer Models Creativeness Data Data Set Development Disease Disease model Distal Ensure Environment Epithelium Evaluation Experimental Designs Feedback Functional disorder Generations Genetic study Genotype Goals Grant Health Homeostasis Hormone Responsive Human Individual Infrastructure Instruction Investigation Journals Kidney Kidney Diseases Leadership Liquid substance Measures Metabolism Methods Mitochondria Modeling Monitor Mus Nephrons Organism Organoids Output Peer Review Pharmaceutical Preparations Physiology Play Process Productivity Protocols documentation Publications Purines Quality Control Reagent Recording of previous events Renal function Reproducibility Research Research Personnel Resolution Resource Development Resource Sharing Resources Rodent Rodent Model Role Saccharomyces cerevisiae Scientist Services Specialized Center System Technology Testing Training Validation Work Yeast Model System Yeasts absorption base biomedical resource cell immortalization cell type design experimental study gene conservation hands on instruction induced pluripotent stem cell innovation kidney cell meetings member metabolomics nephrogenesis new technology next generation proteostasis recruit response to injury sabbatical skills social media solute tool web site

项目摘要

The Model Systems Core continues the Pittsburgh Center for Kidney Research’s rich history of using creative approaches to drive the latest conceptual advances in kidney physiology and pathophysiology. The goal of this biomedical resource Core is to provide a diverse array of innovative model systems that will allow kidney researchers to explore renal development, function, and systemic physiology in normal and disease states. The Core is divided into three Subcores that extend from simple unicellular organisms to complex whole animal models. Taking advantage of the genetically tractable organism S. cerevisiae, the Yeast Models Subcore provides a toolbox for the analysis of conserved genes that play essential roles in kidney physiology and proteostasis. The Epithelial Models Subcore provides access to high fidelity immortalized proximal and distal tubule cell lines, primary cell isolations, and state-of-the art human kidney organoids for the development of disease models, analysis of nephron development, metabolism, function, and response to injury. The Animal Models Subcore provides a broad repertoire of methods to evaluate rodent kidney physiology and pathophysiology, associated cardiovascular variables, mitochondrial health and bioenergetics, and purine metabolomics. Using an infrastructure supported by its established investigators and record of productivity, the Model Systems Core will work seamlessly with the Physiology Core and the KIDNIT Resource Development Core to facilitate discovery. As part of the Consortium, the Core will ensure a facile exchange of tools, technologies, and specialized Center resources to advance kidney research nationwide. Accordingly, the Core welcomes all collaborators, offers mechanisms to promote Subcore tools and reagents, provides hands-on instruction (i.e., mini-sabbaticals), and will facilitate collaborative resource sharing with individual investigators as well as with the Consortium at large. For early-stage investigators and established PIs who are new to kidney research, key resources, training, and collaborative opportunities will be prioritized to cultivate enthusiasm and entry into an active research pipeline. The Core will also provide expert advice and aid in the acquisition of preliminary data to support new and innovative grant applications. Stringent quality control measures will ensure the rigor and reproducibility of Subcore outputs, and a formalized evaluation and feedback process permits regular reassessment of the usefulness of Core resources. By offering impactful state-of-the-art and readily accessible services across diverse model systems, the Core will provide a unique national resource for the kidney research community that will propel team-based investigation and catalyze discovery.

模型系统核心延续了匹兹堡肾脏研究中心使用创意的丰富历史推动肾脏生理学和病理生理学最新概念进展的方法。生物医学资源的核心是提供多样化的创新模型系统，使肾脏研究人员探索正常和疾病状态下的肾脏发育、功能和全身肾脏生理学。核心分为三个子核心，从简单的单细胞生物延伸到复杂的整体动物利用遗传易驯化的酿酒酵母，酵母模型子核心。提供了一个用于分析在肾脏生理学中发挥重要作用的保守基因的工具箱上皮模型子核心提供了高保真度永生化的近端和远端。肾小管细胞系、原代细胞分离和最先进的人类肾脏类器官，用于开发疾病模型，肾单位发育、代谢、功能和损伤反应的分析。 Models Subcore 提供了广泛的方法来评估啮齿动物肾脏生理学和病理生理学、相关心血管变量、线粒体健康和生物能量学以及嘌呤使用由其既定研究人员和生产力记录支持的基础设施，模型系统核心将与生理学核心和 KIDNIT 资源开发无缝协作促进发现的核心。作为联盟的一部分，核心将确保工具的便捷交换，技术和专业中心资源，以推进全国肾脏研究。欢迎所有合作者，提供推广 Subcore 工具和试剂的机制，提供实践机会指导（即小型休假），并将促进与个别研究人员的协作资源共享以及整个联盟的早期研究人员和刚接触肾脏的 PI。将优先考虑研究、关键资源、培训和合作机会，以培养热情和核心还将在收购方面提供专家建议和帮助。支持新的和创新的拨款申请的初步数据将确保严格的质量控制措施。子核心输出的严谨性和可重复性，以及正式的评估和反馈流程允许通过提供有影响力的最新技术并随时重新评估核心资源的有用性。跨不同模型系统的无障碍服务，核心将为肾脏研究社区将推动基于团队的调查并促进发现。