Photochemical Technologies for Improved Glycosylation Reactions

用于改进糖基化反应的光化学技术

• 批准号: 10627108
• 负责人: David Crich
• 金额: $ 21.74万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627108
• 关键词: Address; Alcohols; Anions; Biological; Biological Process; Biology; Carbohydrates; Cations; Chemistry; Communities; Development; Disease; Esters; Fluorides; Glycoconjugates; Glycols; Glycosides; Goals; Health; Human; Ions; Light; Medicine; Methodology; Methods; Oligonucleotides; Oligosaccharides; Peptides; Play; Polysaccharides; Preparation; Protocols documentation; Reaction; Reagent; Regulation; Reproducibility; Role; Source; Specialist; System; Technology; Testing; catalyst; chemical synthesis; design; glycosylation; improved; innovation; irradiation; light emission; new technology; novel; nucleophilic substitution; operation; promoter; scale up

Carbohydrates, glycans, and their conjugates play critical roles in a very broad spectrum of biological processes, many of which are central to human health, development and disease states. The diversity of glycan-dependent biological processes is reflected by the even-more diverse spectrum and complexity of the implicated glycans and glycoconjugates, collectively known as the glycome. The study of glycan dependent biological processes and their eventual regulation and/or exploitation in human medicine is enormously hampered by difficulties in isolating and structurally characterizing meaningful quantities of pure unique glycans, known as homogeneous glycoforms, from biological sources. It is imperative therefore that simple practical technologies for glycan synthesis be developed that combine robustness with ease of operation such that specialist and non-specialists alike will be able to access glycans with relative ease. This proposal aims to develop exactly the kind of scalable robust methodology that is required to address the chemical synthesis of a broad cross section of the glycome. The focus is on the development of direct photochemical methods without the need for external additives thereby reducing the system to its essentials – a glycosyl donor and glycosyl acceptor – and eliminating complexity to the greatest extent possible. The technologies to be developed are designed to employ light-emitting diodes (LEDs) as light sources and ultimately to be applicable under flow conditions so as to enable practical scaled-up syntheses of biologically important glycans.

碳水化合物、聚糖及其结合物在非常广泛的领域中发挥着关键作用 一系列生物过程，其中许多对人类健康至关重要， 聚糖依赖性生物学的多样性。 过程的多样性和复杂性反映了 涉及的聚糖和糖复合物，统称为糖组。 聚糖依赖性生物过程及其最终调节和/或 人类医学的开发由于分离和分离方面的困难而受到极大的阻碍。 在结构上表征有意义数量的纯独特聚糖，称为 因此，必须从生物来源获得同质糖型。 开发将稳健性与 操作简便，专家和非专家都能够访问 该提案旨在开发一种可扩展的聚糖。 解决广泛交叉化学合成问题所需的稳健方法 糖组学部分的重点是直接光化学的发展。 方法无需外部添加剂，从而将系统降低至其 必需品——糖基供体和糖基受体——并消除了复杂性 所开发的技术旨在最大程度地利用。 发光二极管（LED）作为光源并最终适用于流动 条件，以便能够实际放大合成具有生物学意义的化合物 聚糖。