NITRIC OXIDE RELEASING BIOMIMETRIC NANOMATRIX GELF OR ROOT REVITALIZATION

释放一氧化氮的仿生纳米基质凝胶或根部活化

基本信息

批准号：
10623370
负责人：
Kyounga Cheon
金额：
$ 13.29万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2023-08-31
项目状态：
已结题

项目摘要

PROJECT SUMMARY Dental pulp tissue exposed to a mechanical trauma or cariogenic process can result in root canal and/or periapical infections, which can be treated via an endodontic procedure. A regenerative endodontic procedure (REP) attempts to revitalize pulp-dentin tissue from a previously necrotic or inflamed pulp, thus, allowing for continued development of the pulp-dentin structure, particularly for a young immature root. However, the current REP has resulted in unfavorable outcomes, including tooth discoloration, cervical root fractures, inadequate pulp-dentin tissue structure formation, and multiple clinic visits. To tackle the challenges of the current REP, a highly interdisciplinary and innovative strategy is proposed for efficient root canal disinfection and pulp-dentin tissue revitalization using an antibiotics and nitric oxide (NO) releasing biomimetic nanomatrix gel. We hypothesize that the antibiotics and NO releasing nanomatrix gel will demonstrate an efficient antibacterial effect and recruit endogenous stem cells to induce pulp-dentin revitalization, while providing a favorable pulp-dentin tissue mimicking extracellular matrix environment. The proposed strategy has several advantages over the current REP: 1) preventing tooth discolorations by removal of minocycline, 2) preventing cervical root fractures by avoidance of calcium hydroxide, 3) antibacterial effect of the NO, 4) sustained NO release from the nanomatrix gel by enzyme mediated degradation, 5) reducing inflammatory responses by avoidance of reopening root canals and stimulated bleeding, 6) biocompatibility and biodegradability of the nanomatrix gel as a functional extracellular matrix, and 7) less extensive pulp access opening, clinical visits, and restorations with cost-effective materials such as composites resin. Therefore, three specific aims are proposed to evaluate the hypothesis. Specific Aim 1 is to evaluate the anti-biofilm effects of the antibiotics and NO releasing biomimetic nanomatrix gel in clinical samples from the endodontic infection. Specific Aim 2 is to evaluate the cellular effects of the antibiotics and NO releasing biomimetic nanomatrix gel on dental pulp stem cells. Specific Aim 3 is to develop a rat infected tooth model and evaluate the revitalization capacity of the antibiotics and NO releasing biomimetic nanomatrix gel. The outcomes from this proposal will demonstrate the anti-biofilm effect and revitalization potential of the antibiotics and NO releasing nanomatrix gel for the treatment of endodontic infections. Further, the outcomes will also lead to future clinical studies for a young immature root as well as a mature root canal infection with traumatic exposure and bacterial associated cases.

项目概要牙髓组织暴露于机械创伤或致龋过程可能会导致根管和/或根尖周感染，可以通过牙髓手术治疗。再生牙髓手术 (REP) 试图使先前坏死或发炎的牙髓恢复活力，从而允许牙髓-牙本质结构的持续发育，特别是对于年轻的未成熟的牙根。然而，当前 REP 导致了不利的结果，包括牙齿变色、颈根骨折、牙齿不齐等。牙髓-牙本质组织结构的形成，以及多次就诊。为了应对当前 REP 的挑战，提出了高效根管消毒和牙髓牙本质的高度跨学科和创新策略使用抗生素和释放一氧化氮 (NO) 的仿生纳米基质凝胶进行组织再生。我们假设抗生素和释放 NO 的纳米基质凝胶将表现出有效的抗菌作用并招募内源性干细胞诱导牙髓-牙本质再生，同时提供有利的牙髓-牙本质组织模仿细胞外基质环境。与现有策略相比，该策略有几个优点目前的 REP：1) 通过去除米诺环素来预防牙齿变色，2) 预防颈根骨折通过避免使用氢氧化钙，3) NO 的抗菌作用，4) 从纳米基质中持续释放 NO 通过酶介导的降解形成凝胶，5) 通过避免重新打开根管来减少炎症反应和刺激出血，6）纳米基质凝胶作为功能性材料的生物相容性和生物降解性细胞外基质，7) 不太广泛的牙髓通路开口、临床就诊和具有成本效益的修复体复合树脂等材料。因此，提出了三个具体目标来评估该假设。具体目标 1 是评估抗生素和释放 NO 的仿生纳米基质的抗生物膜作用来自牙髓感染的临床样本中的凝胶。具体目标 2 是评估细胞效应牙髓干细胞上的抗生素和 NO 释放仿生纳米基质凝胶。具体目标 3 是发展大鼠感染牙齿模型并评估抗生素和NO释放仿生剂的再生能力纳米基质凝胶。该提案的结果将展示抗生物膜效果和振兴作用抗生素和释放 NO 的纳米基质凝胶治疗牙髓感染的潜力。更远，研究结果还将导致未来针对年轻未成熟牙根和成熟根管的临床研究感染与外伤暴露和细菌相关的病例。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Candida Infection Associated with Salivary Gland-A Narrative Review.

与唾液腺相关的念珠菌感染 - 叙述回顾。

DOI：
发表时间：
2020-12-30
期刊：
影响因子：
0
作者：
Ok, Soo;Ho, Donald;Lynd, Tyler;Ahn, Yong;Ju, Hye;Jeong, Sung;Cheon, Kyounga
通讯作者：
Cheon, Kyounga

Evaluation of the periodontal and pulpal healing of replanted rat molars with doxycycline root conditioning.

用多西环素根调理评估再植大鼠磨牙的牙周和牙髓愈合。

DOI：
发表时间：
2019-06
期刊：
影响因子：
0
作者：
Nam, Ok Hyung;Cheon, Kyounga;Kim, Mi Sun;Lee, Hyo;Choi, Sung Chul
通讯作者：
Choi, Sung Chul

In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration.

脱细胞人牙支架用于牙齿组织再生的体内评价。

DOI：
发表时间：
2021-09-02
期刊：
影响因子：
0
作者：
Kim, Ik;Jeon, Mijeong;Cheon, Kyounga;Kim, Sun Ha;Jung, Han;Shin, Yooseok;Kang, Chung Min;Kim, Seong;Choi, Hyung;Lee, Hyo;Lee, Ko Eun;Song, Je Seon
通讯作者：
Song, Je Seon

Establishment of quantitative indicators for an efficient treatment on masticatory muscle pain.

建立有效治疗咀嚼肌疼痛的定量指标。