Biospecimen Bank

生物样本库

• 批准号: 10242156
• 负责人: Wing C. Chan
• 金额: $ 16.63万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-02 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10242156
• 关键词: 9p24; Animal Model; Area; Biodistribution; Biological Assay; Biological Specimen Banks; Biological Specimen database; Biometry; Biostatistics Core; Blood; Blood Component Removal; Blood specimen; Bone Marrow; Cancer Center; Cell Separation; Cells; Chromosomes; Cities; City of Hope Comprehensive Cancer Center; Classification; Clinical; Collaborations; Collection; Communities; Complement; Complex; Comprehensive Cancer Center; Core Facility; Cytogenetics; Cytometry; DNA Sequencing Facility; Data; Data Set; Databases; Derivation procedure; Development; Diagnosis; Drug Kinetics; Enrollment; Ensure; Funding; Gene Expression Profiling; Genomics; Goals; Histologic; Hodgkin Disease; Human; IL2RA gene; Immune; Immunofluorescence Immunologic; Immunophenotyping; Individual; Informatics; Infrastructure; Institution; Leukapheresis; Link; Lymphoma; Malignant lymphoid neoplasm; Modeling; Molecular; Monitor; Multicenter Studies; Non-Hodgkin' s Lymphoma; Paraffin; Pathologic; Pathology; Patients; Performance; Phenotype; Population; Preparation; Process; Program Research Project Grants; Quality Control; Registries; Research; Research Personnel; Research Project Grants; Research Support; Resources; Sampling; Services; Ships; Specimen; Stains; Time; Tissue Microarray; Tissues; Tumor Tissue; Work; basiliximab; biomarker evaluation; career; chimeric antigen receptor T cells; data repository; design; disease classification; improved; informatics infrastructure; leukemia/lymphoma; member; patient derived xenograft model; programs; prospective; quality assurance; spatial relationship; tumor; tumor microenvironment

Core C is designed to meet the specific needs of the projects and at the same time draw on the strength of the existing Cancer Center (CC) cores without unnecessary duplication of services. This Core will work closely and synergistically with a number of CC Cores as well as other SPORE Cores to provide exceptional support for all the proposed projects and possible inter-SPORE collaborations. The overarching goals of this core is to provide high quality, well annotated and strictly quality controlled biospecimens for the projects, to develop and implement assays needed for the analysis of the biospecimens and to collaborate with Core B to build the informatics infrastructures to facilitate all aspects of research linked to biospecimens. Specific Aim 1 is to provides services in the following areas: (1) acquisition and banking of fresh and paraffin- fixed tissues of lymphoma and blood and bone marrow (BM) samples obtained prospectively and retrospectively from patients enrolled on the COH lymphoma SPORE; we will ensure rapid collection and processing, proper storage and monitoring, and proper annotation and record keeping; (2) comprehensive work-up of lymphoma specimens to ensure correct diagnosis and classification, including immunohistochemical, flow cytometric, molecular pathologic, and cytogenetic studies; (3) performance and assistance in routine histologic and immunohistochemical staining of lymphoma tissues and cells, as well as specialized histologic services such as preparation of multi-tumor blocks or tissue microarrays to the specifications of researchers. Additional specialized services will be performed according to the needs of the projects with the collection of leukapheresis specimens to generate CAR-T cells (Project 1); characterization of complex Hodgkin lymphoma and host cell populations through multispectral multiplexed immunofluorescence (mIF) phenotyping and gene expression profiling (GEP) (Project 3); cytogenetic and genomic characterization of lymphomas and the preparation of samples to generate primary patient-derived xenograft (PDX) non- Hodgkin lymphoma models (Project 4). Because of the complexity of the datasets associated with the functions of Core C, in Specific Aim 2, we will collaborate with Core B (Research Informatics) to design, build, and integrate databases relevant to biospecimens to improve their functionality and connectivity to support SPORE projects and collaborations on other NCI-sponsored projects.

Core C 旨在满足项目的特定需求，同时利用 现有的癌症中心 (CC) 核心，无需不必要的重复服务。该核心将密切合作并 与许多 CC 核心以及其他 SPORE 核心协同作用，为所有用户提供卓越的支持 拟议的项目和可能的 SPORE 间合作。 该核心的总体目标是提供高质量、注释良好且严格的质量控制 项目的生物样本，开发和实施生物样本分析所需的检测方法 并与Core B合作建立信息学基础设施，以促进相关研究的各个方面 到生物样本。 具体目标 1 是在以下领域提供服务：(1) 新鲜石蜡的收购和储存 前瞻性获得的淋巴瘤组织以及血液和骨髓 (BM) 样本 对参加 COH 淋巴瘤 SPORE 的患者进行回顾性研究；我们将确保快速收集和 处理、适当的存储和监控以及适当的注释和记录保存； (2)综合性 对淋巴瘤标本进行检查以确保正确的诊断和分类，包括 免疫组织化学、流式细胞术、分子病理学和细胞遗传学研究； (3) 性能和 协助淋巴瘤组织和细胞的常规组织学和免疫组织化学染色，以及 专门的组织学服务，例如制备多肿瘤块或组织微阵列 研究人员的规格。根据需要，将提供额外的专业服务 收集白细胞分离标本以生成 CAR-T 细胞的项目（项目 1）；的表征 通过多光谱多重免疫荧光观察复杂的霍奇金淋巴瘤和宿主细胞群 (mIF) 表型分析和基因表达谱 (GEP)（项目 3）；细胞遗传学和基因组表征 淋巴瘤和样本制备以产生原发性患者来源的异种移植物（PDX）非 霍奇金淋巴瘤模型（项目 4）。 由于与Core C功能相关的数据集的复杂性，在具体目标2中，我们将 与核心 B（研究信息学）合作，设计、构建和集成与以下相关的数据库 生物样本，以改善其功能和连接性，以支持 SPORE 项目和合作 其他 NCI 资助的项目。