Core B: Scientific Core

核心B：科学核心

• 批准号: 10589820
• 负责人: Glenn C Telling
• 金额: $ 58.57万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-15 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10589820
• 关键词: Address; Animals; Biochemical; Biohazardous Substance; Biological Assay; Biophysics; Body Fluids; Brain; Breeding; Cell Line; Cells; Chronic Wasting Disease; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Collection; Core Facility; Creutzfeldt-Jakob Syndrome; Decontamination; Detection; Detergents; Electron Microscope; Elements; Environment; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes; Equilibrium; Flow Cytometry; Fourier Analysis; Gene Targeting; Gene Transfer Techniques; Genotype; Goals; Housing; Human; Immunohistochemistry; Immunoprecipitation; In Vitro; Individual; Infectious Agent; Infrastructure; Knockout Mice; Maps; Methodology; Methods; Microtus; Modeling; Molecular; Molecular Conformation; Monoclonal Antibodies; Mus; Nature; Participant; Pathogenesis; Perfusion; Polymorph; PrP; PrP variant; PrPSc Proteins; Predisposition; Preparation; Prion Diseases; Prions; Procedures; Property; Proteolysis; Protocols documentation; Reagent; Research Personnel; Resolution; Resources; Rodent Model; Safety; Science; Services; Sheep; Solubility; Source; Specificity; Spectroscopy, Fourier Transform Infrared; Standardization; Structure; System; Techniques; Time; Tissues; Titrations; Transgenic Mice; Transgenic Organisms; Western Blotting; Zoonoses; biophysical techniques; cervid; design; experience; experimental study; improved; innovation; mouse model; novel; operation; programs; skills; transmission process

PROJECT SUMMARY Our broad, long-term objectives are to supply reagents, models and expertise from a centralized Science Core facility to all participants of the Program Project. Core operation represents a balance between providing services based on established procedures, and continued innovation in methods and protocols. Safety is a particular guiding principle of the Core, and is addressed by the many years of experience of Core participants in the study of prion diseases, facilities for safe homogenization and other biohazard operating procedures, prion handling and decontamination. Protocol standardization is another crucial goal, so that all aspects of the Program Project have access to reagents and services produced and operated using consistent methods from a constant, reliable source. The Science Core proposes five Specific Aims to accomplish this goal: Aim I will produce and supply characterized mouse models of prion disease and tissues from those mice; Aim II will supply purified anti-PrP monoclonal antibodies (mAbs) with well-defined properties; Aim III will supply preparations of purified infectious prions; Aim IV will quantify prions using sensitive, high throughput cell-based assays; and Aim V will perform detailed biochemical, biophysical and structural studies of prion strains. The Science Core has shown itself to be capable of adapting to efficiently incorporate elements of previous Core structures as well as providing expanded state of the art facilities, techniques and skills for the entire Program Project. The longstanding experience of the Core Co-Directors, Dr. Telling and Dr. Soto, and Associate Director Dr. Bian, is a significant strength. The Science Core resides in outstanding environments for studies of prion diseases. Studies within individual Projects will be conducted in close collaboration with the Science Core to take advantage of the availability of models and expertise, standardized and consistent methodologies, and uniformly consistent, high quality reagents. The activity of the Science Core is essential for the successful accomplishment of Program goals.

项目概要 我们广泛、长期的目标是提供试剂、模型和专业知识 向计划项目的所有参与者提供集中的科学核心设施。核心运营 代表了根据既定程序提供服务之间的平衡，以及 方法和协议的持续创新。安全是一项特殊的指导原则 核心，并通过核心参与者的多年经验来解决 朊病毒疾病、安全均质化设施和其他生物危害操作的研究 程序、朊病毒处理和净化。协议标准化是另一个问题 关键目标，使计划项目的各个方面都能获得试剂和 使用持续、可靠的一致方法生产和运营的服务 来源。科学核心提出了五个具体目标来实现这一目标： 生产并供应具有特征的朊病毒病小鼠模型以及这些模型的组织 老鼠； Aim II 将提供具有明确定义的纯化抗 PrP 单克隆抗体 (mAb) 特性;目标 III 将提供纯化的传染性朊病毒的制剂；目标 IV 将量化 使用灵敏、高通量的基于细胞的测定法检测朊病毒； Aim V将进行详细的 朊病毒菌株的生物化学、生物物理和结构研究。科学核心有 表明自己能够适应并有效地合并以前的核心元素 结构并提供最先进的设施、技术和技能 对于整个计划项目。核心联合董事 Dr. 的长期经验 Telling 和 Soto 博士以及副主任卞博士是一个重要的力量。科学 核心位于研究朊病毒疾病的优越环境中。研究范围 各个项目将与科学核心密切合作进行，以采取 利用标准化和一致的模型和专业知识的可用性 方法论以及一致的高质量试剂。科学活动 核心对于成功实现计划目标至关重要。