Viral Immunology Core

病毒免疫学核心

基本信息

批准号：
10614636
负责人：
OTTO O YANG
金额：
$ 34.02万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-05-07 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10614636
关键词：
Address Area B-Lymphocytes BLT mice Biological Assay Cell surface Cells Cellular Assay Cellular Immunity Clonality Collaborations Consult Consultations Cytomegalovirus Data Analyses Detection Development Engraftment Enzyme-Linked Immunosorbent Assay Evaluation Experimental Designs Flow Cytometry Fostering Funding Genetic Engineering Goals HIV HIV-1 Humoral Immunities Immune Immune response Immunoglobulin Idiotypes Immunologics Immunology Immunology procedure Individual Infection Inflammatory Intervention Knowledge Laboratories Measurement Methodology Monitor Mutation Pathogenesis Phenotype Plasma Research Research Personnel Running Services Standardization Surface T-Cell Proliferation T-Lymphocyte Testing Transduction Gene Transplantation Viral Viremia antigen-specific T cells antiviral immunity cellular transduction cytokine deep sequencing design gene product immune resistance in vivo integration site latent HIV reservoir mouse model novel operation programs reconstitution success tool vector virology

项目摘要

Core B: Project Summary/Abstract This core will provide support to the overall program by providing centralized viral and immunologic assays, as well as providing scientific guidance to each project. This will streamline, standardize, and integrate experimental methodology between Projects. There are two Aims: Aim 1. To perform immunologic and virologic assays in support of the interventions in the Projects, and develop or optimize novel assays as needed. • Flow cytometric analyses to quantify reconstitution in the huBLT mouse model • Flow cytometric identification of transduced cells expressing surface C46 • Phenotyping of transduced T and B cells • Evaluation of antigen-specific T cell proliferation by flow cytometry • Detection of bNAb expressing B cells by flow cytometry • Quantification of soluble bNAb (Design of quantitative ELISA) • Assessment of bNAb-directed anti-idiotype humoral immunity (Design of an anti-idiotype ELISA) • Monitoring for cellular immunity against transduced gene products • Monitoring for humoral immunity against cell surface transduced gene products C46 and/or huEGFRt (Design of a flow cytometric assay) • Measurement of plasma viremia in the huBLT mouse model • Antiviral assays for cellular immunity in the huBLT mouse model • Antiviral assays for bNAb produced in vivo • Sequencing of HIV-1 env for potential escape mutations • Quantitation of the latent reservoir in the huBLT mouse model • Testing functionality of CMV-specific CAR constructs • Measurement of pro-inflammatory cytokines • Clonal tracking of transduced cells by vector integration site deep sequencing Aim 2. To provide scientific and technical consultation to the Projects. The Core investigators will be available for scientific and technical consultation for each of the scientific projects.

核心 B：项目摘要/摘要该核心将通过提供集中的病毒和免疫学检测来为整个计划提供支持，例如并为每个项目提供科学指导，这将简化、标准化和整合。项目之间的实验方法有两个目标：目标 1. 进行免疫学和病毒学检测以支持项目的干预措施，以及根据需要开发或优化新的检测方法。 • 流式细胞术分析量化 huBLT 小鼠模型的重建 • 表达表面C46 的转导细胞的流式细胞术鉴定 • 转导的T 和B 细胞的表型分析 • 通过流式细胞术评估抗原特异性 T 细胞增殖 • 通过流式细胞术检测表达 bNAb 的 B 细胞 • 可溶性 bNAb 的定量（定量 ELISA 的设计） • bNAb 导向的抗独特型体液免疫的评估（抗独特型 ELISA 的设计） • 监测针对转导基因产物的细胞免疫 • 监测针对细胞表面转导基因产物C46 和/或huEGFRt 的体液免疫（流式细胞术分析的设计） • huBLT 小鼠模型血浆病毒血症的测量 • huBLT 小鼠模型中细胞免疫的抗病毒检测 • 体内产生的 bNAb 的抗病毒检测 • 对 HIV-1 env 进行测序以确定潜在的逃逸突变 • huBLT 小鼠模型中潜在储库的定量 • 测试 CMV 特异性 CAR 构建体的功能 • 促炎细胞因子的测量 • 通过载体整合位点深度测序对转导细胞进行克隆追踪目标 2. 为项目提供科学和技术咨询。为每个科学项目提供科学技术咨询。