Molecular mechanism of natural variation and adaptation of circadian clock

生物钟自然变异与适应的分子机制

基本信息

批准号：
9023044
负责人：
KWANGWON LEE
金额：
$ 42.2万
依托单位：
RUTGERS THE STATE UNIV OF NJ CAMDEN
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-01-01 至 2018-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9023044
关键词：
Alleles American Animal Model Biological Assay Biological Clocks Biological Models Biological Process Biological Rhythm Candidate Disease Gene Chromosomes, Human, Pair 3 Circadian Rhythms Communities Complex Computational Biology Development Dust Environment Evolution Foundations Genes Genetic Genome Genomics Genotype Goals Habitats Health Human Individual Knowledge Life Measures Metabolic Pathway Molecular Nature Neurospora Organism Outcome Outcome Study Pathway Analysis Phenotype Phylogenetic Analysis Play Positioning Attribute Proteomics Regulation Regulator Genes Research Research Personnel Role Rotation Signal Transduction Stress Symptoms Testing Time Tree Bark Variant Work asexual base circadian pacemaker fitness fitness test genome-wide genome-wide analysis improved insight metabolome public health relevance research study tool trait transcriptome

项目摘要

DESCRIPTION (provided by applicant) Most living organisms on earth are adapted to a 24 hr cycling environment due to the rotation of the earth. An organism knows the time of the day and prepares the incoming abiotic and biotic stresses, and also utilizes the expected changes of ambient environment. This time-measuring mechanism in an organism is an important biological function for its survival in nature. The observable biological rhythm with about a 24 hr period is collectively called circadian rhythm, and the underlying molecular mechanism of circadian rhythm is called the circadian clock. It has been suggested in different model organisms that circadian rhythm is a fitness trait. However, we still lack knowledge in regards to how circadian clock achieves the fitness advantage. Researchers in the proposal have identified Continent-Specific-Clock-Variation in Neurospora discreta species complex Phylogenetic Species 4b (PS4b) in strains collected in North American strains in comparison to strains collected from other continents, and proposed to characterize how allelic variations of the adaptive gene causes variation of the clock phenotype to adapt to their unique habitat using genetics, genomics, proteomics, and computational biology tools. They will generate allele-swap strains where their genotype is identical, except the continent-specific allele of the candidate gene. The fitness value of the candidate gene will be tested by measuring the number of progeny under different habitat conditions. The successful outcome of the proposed study will allow us an insight on how a local adaption of circadian rhythm of an organism influences the organism's fitness.

描述（由申请人提供）由于地球的旋转，地球上的大多数生物都适用于24小时的循环环境。可观察到的生物学被称为昼夜节律的昼夜节律，昼夜节律是一种适应性的特征。与从其他大洲的自适应基因原因收集的菌株相比，菌株中的神经孢子dixta物种4b（PS4B），使用遗传学，基因组学，蛋白质组学和计算生物学工具，以适应其独特的栖息地。等位基因等位基因它们的基因型是相同的，除了你除外候选基因的大陆特异性等位基因将通过测量拟议的研究的成功结果测量Willow Usalow Usansight对生物体的局部适应性如何影响生物体的适应性来测试。