Center for Genetic Studies of Drug Abuse in Outbred Rats

近交系大鼠药物滥用基因研究中心

• 批准号: 10613525
• 负责人: Leah Catherine Solberg Woods
• 金额: $ 18.34万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-15 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10613525
• 关键词: Animals; Behavior; Behavioral; Breeding; Chromosome Mapping; Data; Diabetes Mellitus; Drug abuse; Ecosystem; Ensure; Feasibility Studies; Female; Funding; Genes; Genetic; Genetic Drift; Genetic Variation; Genetic study; Goals; Grant; Heritability; Inbred Strains Rats; Inbreeding; Laboratories; Maps; National Institute of Drug Abuse; North America; Obesity; Phenotype; Pilot Projects; Population; Quantitative Trait Loci; Randomized; Rattus; Recombinants; Research; Research Personnel; Research Project Grants; Resources; Substance Use Disorder; United States National Institutes of Health; Universities; Vertebral column; experience; forest; genomic locus; heritable gene drive; male; medical schools; sample fixation; trait

Project Summary/Abstract (Core B: HS Breeding Core) The N/NIH heterogeneous stock (HS) breeding core, under the direction of Dr. Leah Solberg Woods at the Wake Forest University School of Medicine (WFUSM), will provide HS rats to Research Project 1-3. HS animals are outbred from eight inbred rat strains and maintained in a manner that minimizes inbreeding, enabling the fine-mapping of quantitative trait loci (QTL). Dr. Solberg Woods’ laboratory has maintained the HS rat colony since 2006 and hers is the only laboratory in the North America to house this unique resource. Over the past four years as part of the P50 Center for Excellence HS Breeding Core, the HS rat colony has become a national resource. We have sent rats to investigators both inside and outside the P50. Pilot and Feasibility grants funded through the P50 have led to funding for four NIDA-funded U01 grants that use HS rats. The two main goals of this core will be to 1) maintain the HS rat colony in a way that minimizes inbreeding and 2) ship a genetically diverse set of HS rats to Research Projects 1-3. To minimize inbreeding and control genetic drift, as well as to have sufficient rat to send to investigators within the P50 as well as currently funded U01 grants, the HS rat colony will increase from 64 to 92 breeder pairs and will be maintained using a randomized breeding strategy, ensuring that closely related animals are not bred together. The HS Breeding Core will ship HS rats (male and female) four or five times per year to Research Projects 1-3 over a four year period, with Research Project 1 receiving a limited number of rats in year 5. By providing a genetically diverse set of HS rats to Research Projects 1-3, this Core will provide the back-bone for the studies proposed in this P50, namely the genetic fine-mapping and identification of genes involved in behaviors related to substance use disorders.

项目摘要/摘要（核心 B：HS 育种核心） N/NIH 异种种群 (HS) 育种核心，在 Leah Solberg Woods 博士的指导下 维克森林大学医学院 (WFUSM) 将为研究项目 1-3 提供 HS 大鼠。 动物是由八个近交系大鼠进行远交，并以尽量减少近交的方式进行饲养， Solberg Woods 博士的实验室一直维护着 HS。 自 2006 年以来，她的实验室是北美唯一拥有这种独特资源的实验室。 在过去的四年里，作为 P50 HS 育种卓越中心的一部分，HS 大鼠群体已成为 我们已向 P50 内部和外部的调查人员发送了老鼠。 通过 P50 资助的赠款为 NIDA 资助的四项使用 HS 大鼠的 U01 赠款提供了资金。 该核心的主要目标是 1) 以尽量减少近亲繁殖的方式维持 HS 大鼠群体，2) 运送 研究项目 1-3 的遗传多样性 HS 大鼠为了尽量减少近亲繁殖并控制遗传漂移，如 以及有足够的老鼠发送给 P50 内的调查人员以及当前资助的 U01 赠款， HS 大鼠群体将从 64 对饲养员增加到 92 对，并将通过随机饲养进行维持 策略，确保密切相关的动物不会混养在一起，HS 育种核心将运送 HS 大鼠。 （男性和女性）在四年内每年四到五次研究项目 1-3，并进行研究 项目 1 在第 5 年接收有限数量的大鼠。通过提供一组基因多样化的 HS 大鼠 研究项目 1-3，该核心将为 P50 中提出的研究提供支柱，即 基因精细定位和识别涉及药物滥用障碍相关行为的基因。