A Household Yeast Biosensor for Cholera

霍乱家用酵母生物传感器

基本信息

批准号：
9064692
负责人：
VIRGINIA W CORNISH
金额：
$ 65.24万
依托单位：
COLUMBIA UNIV NEW YORK MORNINGSIDE
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-15 至 2020-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9064692
关键词：
Africa African Anabolism Antibodies Benchmarking Binding Biosensor Bioterrorism Cause of Death Centers for Disease Control and Prevention (U.S.)Cholera Cholera Toxin Communities Country Coupling Detection Developing Countries Development Diagnostic Directed Molecular Evolution Disease Dried Yeast Engineering Epidemic Epitopes Equipment Evolution Eye Faculty Feces Feedback Fermentation Food Safety Formulation Freeze Drying Fungal Peptides G Protein-Coupled Receptor Signaling G-Protein-Coupled Receptors Goals Grant Hand Health Hospitals Hour Household Household Products Human Hybrids Infection Laboratories Life Ligands Methods Monitor Noise Parents Peptides Pigments Plants Positioning Attribute Public Health Reaction Time Reagent Reporter Resources Risk Saccharomyces cerevisiae Scientist Series Signal Transduction Specificity System Technology Testing Tomatoes Translating Universities Validation Vibrio cholerae Visual Water Supply Yeasts base clinical practice cost design field study improved killings lycopene metabolic engineering pathogen product development prototype receptor response surveillance network synthetic biology tool water sampling

项目摘要

DESCRIPTION (provided by applicant): Diarrheal diseases are one of the top ten causes of death in developing countries, with V. cholerae being one of the principal bacterial causative agents annually infecting an estimated ~4 million and killing ~120,000 people. Although an easily treatable disease, cholera continues to cause fatal epidemics because in practice clinical detection rarely occurs outside remote hospitals and environmental detection in water supplies remains challenging. The state-of-the- art for cholera diagnostics are purified antibodies used as dipstick tests; unfortunately dipstick tests are prohibitively expensive and unstable for use directly in local communities where cholera is endemic. A new transformative mode of detection will be needed to generate an extremely cheap and widely distributable system for continuous local surveillance of cholera. We sought to overcome current obstacles to local diagnostics by using existing synthetic biology components to craft a new class of cheap and simple diagnostic built around freeze-dried yeast. We avoid the need for expensive equipment and additional reagents by engineering yeast to produce the red tomato pigment lycopene in response to the cholera pathogen. We envision that engineered baker's yeast can be freeze-dried and then globally distributed to provide an inexpensive, safe, simple and reliable tool for monitoring of cholera in communities at risk for infection. If successful, this yeast platform will be easily redeployable for the detection of other pathogens.

描述（由适用提供）：腹泻疾病是发展中国家死亡的十大死亡原因之一，霍乱葡萄球菌是每年受到约400万感染的主要细菌之一，造成约12万人感染约120,000人。尽管一种易于治疗的疾病，但霍乱仍会继续引起致命发作，因为在实践中，临床检测很少发生在偏远医院外，并且在供水中的环境检测仍然受到挑战。霍乱诊断的最先进的纯化抗体用作插件测试；不幸的是，禁止使用量强壮的测试昂贵且不稳定，以直接在霍乱的霍乱中使用。将需要一种新的变革性检测方式，以生成一种极为便宜且分布广泛的系统，以持续对霍乱的局部监视。我们可以通过使用现有的合成生物学组件来制作一类新的廉价和简单的诊断，以克服当前诊断障碍的障碍。我们通过工程酵母来避免需要昂贵的设备和其他试剂，以响应霍乱病原体生产红色番茄色素番茄红素。我们设想，工程化的面包师的酵母可以被冷冻干燥，然后在全球范围内分发，以提供廉价，安全，简单和可靠的工具，用于监测处于感染风险的社区中的霍乱。如果成功，该酵母平台将很容易重新部署以检测其他病原体。