Stony Brook University Laboratory for Comparative Medicine to Support Pandemic Preparedness

石溪大学比较医学实验室支持流行病防范

基本信息

批准号：
10611662
负责人：
David G Thanassi
金额：
$ 293.31万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-16 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10611662
关键词：
2019-nCoV Adenoviruses Aerosols American Animal Model Animals Applied Research Area Basic Science Biological Products Biotechnology Bunyavirales COVID-19 COVID-19 therapeutics COVID-19 vaccine Containment Coronavirus Custom Dengue Virus Detection Disease Duct (organ) structure Faculty Family Family Picornaviridae Flaviviridae Francisella tularensis Funding Future Gases HIV Hantavirus High temperature of physical object Immune response Immunotherapy Individual Infection Influenza A virus Investigation Laboratories Measures Medicine Mycobacterium tuberculosis New York Pathogenesis Persons Phase Play Plumbing Population Powassan virus Preventive Process Publications Quantitative Reverse Transcriptase PCR RNA Viruses RNA analysis Recording of previous events Research Research Activity Research Personnel Resources Reverse Transcription Rickettsia Rodent Role Security Steam System Therapeutic Time Translational Research Treatment Efficacy Tuberculosis Tularemia Universities Viral Virulent Virus Water Work Zika Virus Zoonoses animal imaging anti-viral efficacy antimicrobial antiviral drug development biosafety level 3 facility combat comparative cost design emerging pathogen equipment acquisition exhaust experience flexibility fluorescence activated cell sorter device heat exchanger improved infectious disease model interest metropolitan microbial operation pandemic disease pandemic preparedness pathogen pathogenic bacteria pathogenic fungus pathogenic microbe pathogenic virus pre-clinical pre-clinical research programs spotted fever tool virology

项目摘要

PROJECT SUMMARY/ABSTRACT The Laboratory for Comparative Medicine (LCM) at Stony Brook University is a recently constructed, state-of- the-art animal biosafety level 3 (ABSL3) facility. The LCM is actively engaged in basic, translational, and preclinical research on SARS-CoV-2, the viral agent of the current COVID-19 worldwide pandemic. Research in the LCM serves multiple individual investigators and groups, both within and outside of Stony Brook University, and includes commercial as well as academic interests. Stony Brook has a long history of excellence in microbial pathogenesis research, including studies on pandemic potential viruses of concern in the families Coronaviridae (SARS-CoV-2), Bunyavirales (Hantaviruses), Flaviviridae (Dengue, Zika, and Powassan viruses), and Picornaviridae (Poliovirus). Stony Brook virologists are also conducting ongoing studies in Human Immunodeficiency Virus and Influenza A Virus. Our research over the past five years has covered three different RNA virus families relevant under the American Pandemic Preparedness/pandemic preparedness effort, and our cumulative experience covers four of the viral families of concern. In addition to viruses of concern, active research at Stony Brook and in the LCM includes work on the highly virulent bacterial agents Rickettsia spp. of the spotted fever group, Francisella tularensis, a Select Agent and cause of the zoonotic disease tularemia, and Mycobacterium tuberculosis, causative agent of tuberculosis. To improve facility operations and enhance ongoing and future research in the LCM, we are requesting four upgrades to the LCM: 1) Replacement of the high temperature hot water unfired steam boilers and conversion of the high temperature and hot water systems; 2) Purchase and installation of a washer for animal cages and racks; 3) Purchase and installation of a fluorescence-activated cell sorter and custom biosafety enclosure; and 4) Acquisition of a real-time, quantitative reverse-transcription PCR machine. The requested upgrades will support and improve pandemic preparedness-relevant research in the LCM, advance studies on highly virulent and emerging pathogens, and provide enhanced resources not only to Stony Brook researchers, but also to State and regional efforts to combat current and future pandemic threats. The requested improvements will support the development of antiviral programs and antimicrobial preventive and therapeutic measures.

项目概要/摘要石溪大学比较医学实验室 (LCM) 是最近建成的、最先进的最先进的动物生物安全3级（ABSL3）设施。 LCM 积极致力于基础、转化和针对 SARS-CoV-2（当前 COVID-19 全球大流行的病毒病原体）的临床前研究。研究 LCM 为石溪内外的多名个人调查员和团体提供服务大学，包括商业和学术兴趣。石溪有着悠久的历史卓越的微生物发病机制研究，包括对潜在大流行病毒的研究冠状病毒科 (SARS-CoV-2)、布尼亚病毒科 (汉坦病毒科)、黄病毒科 (登革热病毒、寨卡病毒和 Powassan 病毒）和小核糖核酸病毒科（脊髓灰质炎病毒）。石溪病毒学家也在进行持续的研究研究方向为人类免疫缺陷病毒和甲型流感病毒。我们过去五年的研究涵盖了美国流行病防范/流行病相关的三种不同的 RNA 病毒家族准备工作，我们积累的经验涵盖了四个值得关注的病毒家族。此外石溪分校和 LCM 的积极研究包括针对高毒力病毒的研究细菌制剂立克次体属。斑点热病群的土拉弗朗西斯菌，一种选择病原体和病因人畜共患病兔热病和结核病病原体结核分枝杆菌。改善设施运营并加强 LCM 中正在进行和未来的研究，我们请求进行四次升级 LCM：1）更换高温热水无火蒸汽锅炉和改造高温热水锅炉温度和热水系统； 2）购买并安装动物笼、架清洗机； 3）购买并安装荧光激活细胞分选仪和定制生物安全外壳；和 4) 购置实时定量逆转录 PCR 机。所请求的升级将支持和改进 LCM 中与流行病防范相关的研究，推进高毒力研究和新出现的病原体，不仅为石溪研究人员提供更多资源，还为国家和地区努力应对当前和未来的流行病威胁。所要求的改进将支持制定抗病毒计划以及抗菌预防和治疗措施。