Molecular Cytogenetics

分子细胞遗传学

基本信息

批准号：
9102926
负责人：
STEPHEN G EMERSON
金额：
$ 9.93万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9102926
关键词：
Adopted Base Pairing Bioinformatics Budgets Cancer Center Cancer Center Support Grant Chromosome abnormality Chromosomes Collaborations DNA DNA Methylation Data Development Epigenetic Process Equipment Evaluation Fees Fluorescent in Situ Hybridization Formalin Funding G-Banding Goals Gold Human Image Karyotype Karyotype determination procedure Malignant Neoplasms Microscopy Modality Modification Molecular Molecular Cytogenetics Mus Normal tissue morphology Paraffin Embedding Pilot Projects Process Research Research Design Research Personnel Research Project Grants Resolution Resource Sharing Rodent Running Sampling Services Spectral Karyotyping System Technical Expertise Technology Testing Time Tissues Tumor Cell Line Tumor Tissue bisulfite sequencing cancer cell cancer genome experience flexibility member methylation pattern programs tool

项目摘要

The Molecular Cytogenetics & Epigenetics Shared Resource (MCESR) is a fully HICCC-administered and operated facility, which provides technical expertise and equipment necessary for identifying chromosomal aberrations in cancer cells, and specialized high throughput targeted bisulfite sequencing (bis-seq) to determine DNA methylation patterns at base-pair resolution in cancer and normal tissues. Molecular cytogenetic services include conventional G-banding karyotype, fluorescence in situ hybridization (FISH), spectral karyotyping (SKY) in human and mouse-derived tumor cell lines and tissues. These modalities are all integrated with a dedicated microscopy system for automated image capture and analysis. Current epigenetics services are focused on sequencing of bisulfite-converted DNA (bIs-seq) in tumor and normal tissues with high sample and amplicon throughput, using a Fluidigm AccessArray to MISEQ pipeline. We provide the necessary bioinformatics support both from our own staff and through our collaboration with the Bioinformatics SR (BISR). We provide flexible help to HICCC investigators with study design for molecular cytogenetics and epigenetics. Our development activities include evaluation and incorporation of emerging chromosome and DNA methylation technologies, including ways to generate high quality molecular data from formalin-fixed paraffin-embedded (FFPE) material. During this last 5 years of CCSG funding, 90% of the investigators using the Molecular Cytogenetics SR have been Cancer Center members. The total operating budget ofthe facility is $317,686 ofwhich we are requesting $66,030 from the CCSG

分子细胞遗传学和表观遗传学共享资源（MCESR）是完全HICCC管理的，并且操作的设施，该设施提供了识别染色体所需的技术专长和设备癌细胞的像差，以及专门的高吞吐量靶向亚硫酸盐测序（BIS-SEQ）确定癌症和正常组织基础对分辨率下的DNA甲基化模式。分子细胞遗传学服务包括常规的G带核型，荧光原位杂交（FISH），在人和小鼠衍生的肿瘤细胞系和组织中的光谱核分型（天空）。这些方式是所有这些都与专用显微镜系统集成，以进行自动图像捕获和分析。当前的表观遗传学服务的重点是肿瘤和正常的甲硫酸含硫酸含量转换DNA（BIS-SEQ）的测序具有高样品和扩增子吞吐量的组织，使用流体访问权限到Miseq Pipeline。我们提供我们自己员工以及通过与我们的合作提供必要的生物信息学支持生物信息学SR（BISR）。我们为HICCC研究人员提供了灵活的帮助，具有分子研究设计细胞遗传学和表观遗传学。我们的开发活动包括评估和合并新兴染色体和DNA甲基化技术，包括生成高质量分子数据的方法从福尔马林固定石蜡填充（FFPE）材料中。在CCSG资金的最后5年中，有90％使用分子细胞遗传学SR的研究人员是癌症中心成员。该设施的总运营预算为317,686美元，我们要求CCSG $ 66,030