Musculoskeletal Structure and Strength Core

肌肉骨骼结构和核心力量

基本信息

批准号：
10602565
负责人：
MATTHEW J SILVA
金额：
$ 14.35万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-04-15 至 2024-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10602565
关键词：
Address Adherence Animal Model Autopsy Biology Biomechanics Cartilage Communities Data Development Dual-Energy X-Ray Absorptiometry Ensure Equipment Evaluation Exhibits Fees Fostering Fracture Funding Future Goals Human Resources Image Image Enhancement Individual Investments Manuscripts Measures Mechanics Medicine Methods Microscope Morphology Muscle Muscle Tension Musculoskeletal Musculoskeletal structure Outcome Productivity Property Protocols documentation Publications Reporting Research Research Personnel Resolution Resources Roentgen Rays Schedule Services Structure Techniques Tendon structure Testing Tissues Training Training Programs Universities Washington animal tissue bone cellular imaging contrast enhanced cost effective functional outcomes imaging approach in vivo instrument long bone mechanical properties member microCT musculoskeletal imaging notch protein novel strategies online resource programs radiological imaging response skeletal disorder spine bone structure submicron

项目摘要

Summary – Resource Core B Measures of structure and strength are essential when assessing functional outcomes in animal models related to musculoskeletal biology and medicine. Evaluating these outcomes requires specialized equipment and expertise, which is impractical and inefficient to recreate in multiple labs. In response to this need, we established the Musculoskeletal Structure and Strength Core. This multifaceted resource supports our Research Community in understanding and implementing techniques to evaluate structure and mechanical properties of musculoskeletal tissues (bone, cartilage, disc, muscle, tendon) derived from animal models. We have had a strong impact. Since 2009, we have provided billable services to 94 unique investigators, who have reported structure or strength data in 176 manuscripts which have been cited 3266 times. The need for these services remains high, with 57 current and projected future users. In order to sustain and extend this resource going forward, we propose two Specific Aims. Aim 1 – Imaging: Maintain protocols and equipment, provide training and technical support, and perform imaging of musculoskeletal structures and tissues from animal models generated by Research Community investigators. Routine techniques include plane radiography, dual-energy X-ray absorptiometry (DXA), and micro-computed tomography (microCT), each available post mortem or in vivo. We have recently developed methods for contrast-enhanced imaging; we will make these available to our users and will continue to develop new approaches for imaging non-mineralized tissues (cartilage, disc, muscle, tendon). We will present regular seminars on imaging and strengthen our partnership with the Center for Cellular Imaging. Aim 2 – Mechanical Testing: Perform mechanical testing to assess the functional properties of musculoskeletal structures and tissues from animal models generated by Research Community investigators. Standard testing methods include long-bone bending, vertebral compression, tendon and tendon-to-bone tensile testing, and bone microindentation. Newly established methods include passive and active muscle tension testing and notched bending for fracture toughness. We will present seminars and provide training and enrichment to enhance understanding of biomechanics among Center investigators. In summary, Core B will provide critical support to members of our large Research Community to enable cost-effective utilization of methods to quantify morphology and mechanical properties from musculoskeletal tissues (bone, tendon, muscle, cartilage, disc). Through our efforts we will enhance research productivity and rigor, and provide access to state-of-the-art and emerging techniques.

摘要 – 资源核心 B 在评估动物模型中的功能结果时，结构和强度的度量至关重要与肌肉骨骼生物学和医学有关。设备和专业知识，在多个实验室中重新创建的设备和专业知识是有效的。需要，我们建立了肌肉骨骼结构和强度核心核心核心核心核心核心核心核心核心核心核心核心。我们理解和实施技术以评估结构和肌肉骨骼组织（骨，软骨，椎间盘，肌肉，肌腱）的机械性能动物模型。调查人员，Ho Ho Habe在176篇手稿中报告了结构或力量数据，这些手稿被引用了3266 时间。维持并扩展了这一资源，我们提出了两个特定目标。协议和设备，提供培训和技术支持，并执行肌肉的成像研究社区研究人员产生的动物模型的结构和组织。技术包括平面射线照相，双能X射线吸收法（DXA）和微型计算断层扫描（Microck），每个可用的验尸或体内。对比增强成像；成像非矿化组织的方法（软骨，椎间盘，肌肉，肌腱）。关于成像的研讨会和加强与蜂窝成像中心的合作伙伴关系。机械测试：评估肌肉骨骼的功能特性的执行测试研究社区研究者产生的动物模型的结构和组织。测试方法包括长骨弯曲，椎骨压缩，肌腱和肌腱到骨拉伸测试和骨显微识别。测试和弯曲韧性的弯曲。富集以增强中心研究人员的生物力学。将为我们的研究社区成员提供关键的支持，以实现具有成本效益的利用量化肌肉骨骼组织形态和机械性能的方法（骨骼，肌腱，肌肉，软骨，光盘）。访问最新的技术和新兴技术。