Defining the microtubule motors which drive the uncoating and trafficking of HIV

定义驱动 HIV 脱壳和运输的微管马达

基本信息

批准号：
9099734
负责人：
Edward M Campbell
金额：
$ 31.05万
依托单位：
LOYOLA UNIVERSITY CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-06-25 至 2020-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9099734
关键词：
Adenoviruses Affect Amino Acids Antiviral Therapy Automobile Driving Binding Biochemical Biological Assay Capsid Capsid Proteins Cell Nucleus Cell physiology Cells Coin Collaborations Complex Cyclosporine Data Dependency Docking Dynein ATPase Enzymes Event Family member Future Grant HIV HIV-1 Health Host Defense Image Imaging Techniques In Situ In Vitro Individual Infection Integration Host Factors Interphase Cell Kinesin Label Lead Life Cycle Stages Light Measures Mediating Methods Microtubule-Associated Proteins Microtubules Modeling Motor Mutation Nuclear Pore Complex Nuclear Pore Complex Proteins Pathway interactions Play Primate Lentiviruses Process Reverse Transcription Role Series Stretching Testing Viral Viral Genome Virion Virus Diseases Virus Replication Work base fluorescence imaging high throughput analysis in vivo interest knock-down live cell imaging novel therapeutic intervention pressure prevent research study spatiotemporal trafficking

项目摘要

DESCRIPTION (provided by applicant): HIV-1 uncoating is a poorly understood field and strengthening the idea behind this mechanism will add a strong base to the viral life cycle and interesting targets for antiviral therapy. Our previous work on the role of microtubules during HIV-1 uncoating has provided valuable information to the field of trafficking and HIV-1 uncoating. Our preliminary work supports a strong dependency of several microtubule associated proteins (MAPs) during HIV-1 uncoating and involvement of a variety of other host factors in this process. With this, we propose three specific aims to explore the role of these MAPs during HIV-1 uncoating. Our first aim would be to decipher a mechanism by which these MAPs control viral uncoating. In the second aim, we would further characterize how the viral core trafficking is controlled by these MAPs and in the last aim, we would define the direct interactions between MAPs and the viral core which contribute to HIV-1 uncoating and trafficking. Our expertise and collaborations would help achieve these specific aims and show how the microtubule trafficking machinery plays an important role during HIV-1 uncoating. Findings from these proposed aims will provide critical understanding to the molecular interactions which drive HIV-1 uncoating and trafficking and would help identify better candidates for future antiviral therapy.

描述（由申请人提供）： HIV-1的脱涂是一个贫困的领域，强度指示了一些抗抗病治疗的方法。 Sverotubule的强依赖性在此过程中涉及其他宿主的蛋白质（地图）hiv-1涉及其他宿主。进一步的特征是如何通过这些地图控制病毒核心贩运，在最后一个目标中，我们将定义地图和病毒核心之间的直接核心核心，这些核心核心将有助于实现这些特定目标并显示微管运输方式机械在HIV-1脱落和贩运过程中起着至关重要的作用。