Kansas Center for Metabolism and Obesity REsearch (KC-MORE) - Metabolism Core

堪萨斯代谢与肥胖研究中心 (KC-MORE) - 代谢核心

• 批准号: 10598029
• 负责人: Paige C Geiger
• 金额: $ 29.71万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10598029
• 关键词: Adipocytes; Adipose tissue; Behavior; Behavioral; Bioenergetics; Biological Models; Body Composition; Body measure procedure; Carbohydrates; Cell Size; Cells; Cellular Metabolic Process; Chronic; Chronic Disease; Core Facility; Coupling; Diet; Dietary intake; Disease; Energy Intake; Energy Metabolism; Environmental Risk Factor; Equipment; Exercise; Fasting; Fatty Acids; Fatty acid glycerol esters; Food Intake Regulation; Genus Hippocampus; Glucose; Homeostasis; Human; Impairment; Individual; Insulin Resistance; Isotope Labeling; Isotopes; Kansas; Liver; Macronutrients Nutrition; Measurement; Measures; Mediating; Metabolic; Metabolic Diseases; Metabolic dysfunction; Metabolism; Methodology; Mission; Mitochondria; Mus; Muscle; Obesity; Obesity associated disease; Outcome Measure; Oxygen Consumption; Palmitates; Pancreas; Participant; Pathologic; Physical activity; Play; Pre-Clinical Model; Predisposition; Rattus; Reactive Oxygen Species; Research; Research Personnel; Resources; Rodent; Rodent Model; Role; Sampling; Scientist; Services; Sleep; System; Temperature; Testing; Thermogenesis; Time; Time-restricted feeding; Tissues; Tracer; Treatment Protocols; Weight Gain; design; diet and exercise; dietary manipulation; energy balance; human model; in vivo; insulin tolerance; metabolic abnormality assessment; metabolic phenotype; new therapeutic target; obesity development; oxidation; pre-clinical; tool; translational study

METABOLISM CORE: PROJECT SUMMARY Metabolism plays a fundamental role in the development of obesity and the pathological consequences of obesity that lead to chronic disease. Dysregulation of energy balance and impaired storage or oxidation of substrates are fundamental features of developing obesity and metabolic disease. Metabolic dysregulation is driven by both alterations in cellular mitochondrial function and disruptions in whole body metabolic homeostasis. Therefore, metabolic research that connects cellular mitochondrial energetics to whole body in vivo substrate metabolism and energy expenditure is critical for understanding mechanisms underlying metabolic dysfunction, obesity, and obesity-related disease states. The Metabolism (MET) core will provide the Kansas Center for Metabolism and Obesity REsearch (KC-MORE) with expertise, methodologies, and equipment for the study of “metabolism” from the cell to the whole body. Core missions of the MET core will be to develop a new central MET core facility for pre-clinical Models (rodents) that provides a host of key measurements, allowing for thorough metabolic phenotyping. These approaches will include expertise and equipment to quantify real time measures of in-vivo energy metabolism, substrate metabolism, food intake regulation, and glucose/insulin tolerance testing along with non-invasive measures of body composition in pre-clinical rodent models (rat and mice). A second core feature of the MET core will be to provide resources to investigators to quantify mitochondrial energetics in cells and tissues from both rodent and human samples. Finally, the MET core will also have the capability to perform metabolic isotope methodologies (isotopic labeled glucose, fatty acids, etc.) for use in humans or rodent models (in-vivo or ex-vivo) to quantify overall metabolic flux. In summary, the MET core services will be organized to provide state-of-the art core services to investigators studying metabolism in pre-clinical rodent model systems and to translational measures of mitochondrial energetics and stable metabolic isotopes for use in cells, tissues, rodents, and human participants. The MET core is also designed to largely parallel the capabilities of the Human Energy Balance core so that scientists in the KC-MORE can conduct translational studies in both rodents and humans.

新陈代谢核心：项目摘要 代谢在肥胖的发生和肥胖的病理后果中发挥着基础作用 导致慢性疾病的能量平衡失调和底物储存或氧化受损。 肥胖和代谢疾病的基本特征是由两者驱动的。 细胞线粒体功能的改变和全身代谢稳态的破坏因此， 将细胞线粒体能量学与全身体内底物代谢联系起来的代谢研究 能量消耗对于理解代谢功能障碍、肥胖和肥胖的潜在机制至关重要 代谢（MET）核心将为堪萨斯代谢中心提供服务。 肥胖研究 (KC-MORE) 拥有用于研究“代谢”的专业知识、方法和设备 MET 核心的核心任务是开发一个新的中央 MET 核心设施。 临床前模型（啮齿动物）提供大量关键测量，允许彻底的代谢 这些方法将包括量化体内实时测量的专业知识和设备。 能量代谢、底物代谢、食物摄入调节和葡萄糖/胰岛素耐量测试 在临床前啮齿动物模型（大鼠和小鼠）中对身体成分进行非侵入性测量第二个核心。 MET 核心的特点是为研究人员提供量化细胞中线粒体能量的资源 最后，MET 核心也将具有执行任务的能力。 用于人类或啮齿动物模型的代谢同位素方法（同位素标记的葡萄糖、脂肪酸等） （体内或离体）以量化总体代谢通量 总之，MET 核心服务将被组织为： 提供最先进的核心服务来检查临床前啮齿动物模型系统中的代谢研究 以及用于细胞、组织的线粒体能量学和稳定代谢同位素的转化测量， MET 核心的设计也很大程度上与人类的能力相似。 能量平衡核心，使 KC-MORE 的科学家能够在啮齿类动物和 人类。