Writers and Erasers of Ubiquitin Moieties in Control of Cell-to-Cell Transport in Plants

控制植物细胞间运输的泛素部分的书写者和擦除者

• 批准号: 10593120
• 负责人: VITALY H CITOVSKY
• 金额: $ 39.88万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-16 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10593120
• 关键词: Address; Agrobacterium; Bacteria; Biology; Carrier Proteins; Cells; Gene Expression Regulation; Genes; Genetic Transformation; Goals; Knowledge; Laboratories; Life Cycle Stages; Mammals; Molecular; Movement; Nanotubes; Nucleic Acids; Pathway interactions; Plant Model; Plant Viruses; Plants; Plasmodesmata; Proteins; Regulation; Research; Role; Transport Process; Ubiquitin; Viral; cell motility; epigenetic regulation; histone modification; innovation; intercellular connection; macromolecule; pathogen; plant virus movement protein; programs; response; tool; ubiquitin isopeptidase; ubiquitin-protein ligase

PROJECT SUMMARY Perhaps one of the most intriguing, yet least studied, aspects of macromolecular transport is traffic through intercellular connections. These connections are termed tunneling nanotubes in mammals and plasmodesmata (Pd) in plants. Whereas both mammalian and plant viruses utilize cell-to-cell transport pathways, the first such capability was identified for movement proteins (MPs) of plant viruses, the transport of which via Pd represents a conceptual paradigm for intercellular traffic of macromolecules. Whereas the main research effort in the field, including our lab, has been focused on the mechanism of MP cell-to-cell movement, the important question of how the movement mechanism is controlled remained largely unaddressed. The proposed research program aims to begin filing this gap in our knowledge. Research in my laboratory focuses on three diverse aspects of plant biology and plant-pathogen interactions: (i) cell-to-cell transport of the movement protein (MP) of the model plant virus TMV; (ii) bacterium-to-plant cell transport of proteins and nucleic acids during genetic transformation by Agrobacterium; and (iii) epigenetic regulation of gene expression, including the pathogen response genes, by histone modifications. Recently, all three projects converged on the regulatory roles of ubiquitin writers (E3 ligases) and erasers (deubiquitinases) in the plant life cycle in general, and in the MP cell-to-cell transport in particular. Specifically, our findings suggest that ubiquitin writers and erasers dynamically control two major aspects of the MP cell- to-cell transport process: cargo stability/availability and assembly/disassembly of the Pd transport machinery. The proposed program leverages the wealth of molecular tools and experimental approaches that we have developed along the years to study cell-to-cell transport of plant viruses and roles of protein ubiquitylation/deubiquitylation in plant-pathogen interactions to address a new (and, so far, completely unresearched) level of regulation of intercellular transport through Pd by reversible action of ubiquitin writers and erasers of the plant cell. We believe that this goal is innovative and likely to be transformative also for studies of transport of other proteins, viral and plant, through Pd.

项目概要 也许是大分子最有趣但研究最少的方面之一 运输是通过细胞间连接的交通。这些连接称为隧道 哺乳动物中的纳米管和植物中的胞间连丝（Pd）。而哺乳动物和植物 病毒利用细胞间的运输途径，第一个这种能力被确定为 植物病毒的运动蛋白（MP），其通过 Pd 的运输代表了 大分子细胞间运输的概念范式。鉴于主要研究 包括我们实验室在内的该领域的工作重点是 MP 细胞间的机制 运动，如何控制运动机制的重要问题仍然存在 很大程度上没有得到解决。拟议的研究计划旨在填补我们的这一空白 知识。我实验室的研究重点是植物生物学的三个不同方面 植物-病原体相互作用：（i）模型运动蛋白（MP）的细胞间运输 植物病毒TMV； (ii) 遗传过程中蛋白质和核酸从细菌到植物细胞的运输 通过农杆菌转化； (iii) 基因表达的表观遗传调控，包括 通过组蛋白修饰来识别病原体反应基因。近期，三个项目汇聚一堂 关于植物中泛素书写器（E3 连接酶）和擦除器（去泛素酶）的调节作用 一般而言，生命周期，特别是 MP 细胞间运输。具体来说，我们的发现 表明泛素写入器和擦除器动态控制 MP 细胞的两个主要方面 - 到单元运输过程：货物稳定性/可用性以及 Pd 的组装/拆卸 运输机械。拟议的计划利用了丰富的分子工具和 我们多年来开发的用于研究细胞间运输的实验方法 植物病毒的作用以及蛋白质泛素化/去泛素化在植物-病原体相互作用中的作用 解决细胞间质的新的（迄今为止，完全未经研究的）调节水平 通过植物细胞泛素书写器和擦除器的可逆作用通过 Pd 进行运输。我们 相信这个目标是创新的，并且可能对运输研究产生变革 其他蛋白质，病毒和植物，通过 Pd。