More Than Mechanical Retention: Characterization of Lactobacillus Clinical Strains Using In Vitro Models

不仅仅是机械保留：使用体外模型表征乳酸菌临床菌株

• 批准号: 10593599
• 负责人: ZEZHANG TOM WEN
• 金额: $ 22.28万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10593599
• 关键词: Acids; Adopted; Amino Acids; Anabolism; Bacteria; Bacterial Adhesins; Biological Assay; Caring; Clinical; Code; Collection; Communicable Diseases; Communities; Complex; Consumption; Cues; Dental Plaque; Dental caries; Development; Disease; Ecology; Emergency Situation; Etiology; Foundations; Functional disorder; Genes; Glucans; Glucosyltransferase; Goals; Health; Hour; Hydrogen Peroxide; In Vitro; Indigenous; Inverse Polymerase Chain Reaction; Investigation; Knowledge; Lactobacillus; Lactobacillus casei; Lead; Lesion; Libraries; Link; Mechanics; Mediating; Metabolic; Microbial Biofilms; Modeling; Mutagenesis; Oral; Oral cavity; Outcome; Pathogenicity; Peptides; Polysaccharides; Production; Proteins; Role; Saliva; Site; Source; Streptococcus mutans; Sucrose; Surface; Testing; antagonist; bacterial metabolism; bacteriocin; cost; dental biofilm; dysbiosis; genetic regulatory protein; in vitro Model; insight; member; metabolomics; microbiota; mutant; novel; novel strategies; pathogen; productivity loss; response; stressor; sugar; tooth surface

SUMMARY Dental caries remains among the most prevalent infectious disease worldwide, costing USA tens of billions of dollars annually in associated care and productivity losses. Dental caries is a result of imbalance of the indigenous microbiota on the tooth surface known as dental plaque, and the acidic metabolites of bacterial metabolism are directly linked to the disease development. It is well understood that conditions like continuous consumption of fermentable sugars, especially sucrose and saliva deficiency favor the emergency of a highly acidogenic and aciduric plaque microbiota, which include Streptococcus mutans and lactobacilli. Lactobacillus (Lb) sp. were the first implicated in dental caries over a century ago, but major gaps remain in knowledge concerning how the different Lb sp. establish and persist in the plaque microbiota and promote the development of carious lesions. In this study, a collection of hundreds of different clinical Lb isolates will be examined using well established in vitro models to elucidate how major groups of Lb sp. colonize and establish on a surface; how S. mutans and environmental conditions may modulate the ability of major Lb sp. to establish and compete in a mixed-species consortium; and how interactions between S. mutans and major Lb sp. influence the community dynamics under conditions typical of the oral cavity. It is highly anticipated that the results from this study will lead to novel insights on each of these aspects and the roles of oral Lb in plaque microbiota and dental caries. Together, the information derived from this study should also serve as a foundation for further investigation on the pathophysiology of oral Lb, the plaque biofilm ecology, and the development of novel strategies against dental caries and other conditions where dysbiotic microbiota is a major underlying factor.

概括 龋齿仍然是全球最流行的传染病之一，给美国造成数百亿美元的损失 每年相关的护理和生产力损失。龋齿是牙齿不平衡的结果 牙齿表面的固有微生物群（称为牙菌斑）和细菌的酸性代谢物 代谢与疾病的发生有直接关系。众所周知，像这样的条件 持续消耗可发酵糖，特别是蔗糖和唾液缺乏有利于 高产酸和尿酸菌斑微生物群的紧急情况，其中包括变形链球菌和 乳酸杆菌。乳杆菌 (Lb) sp.一个多世纪前首次与龋齿有关，但主要 关于不同 Lb sp 的知识仍然存在差距。建立并坚持牌匾 微生物群并促进龋齿的发展。在这项研究中，收集了数百个 将使用完善的体外模型对不同的临床 Lb 分离株进行检查，以阐明其主要作用 Lb sp 组。在表面上殖民并建立；变形链球菌和环境条件如何可能 调节主要 Lb sp 的能力。建立混合物种联盟并参与竞争；以及如何 S. mutans 和主要 Lb sp 之间的相互作用。影响条件下的群落动态 口腔的典型特征。预计这项研究的结果将带来新的见解 这些方面以及口腔 Lb 在牙菌斑微生物群和龋齿中的作用。在一起， 从这项研究中获得的信息也应作为进一步调查的基础 口腔 Lb 的病理生理学、菌斑生物膜生态学以及针对其的新策略的开发 龋齿和其他以失调微生物群为主要潜在因素的疾病。