Improved scalability, sensitivity, and interpretability of pathogen detection, including SARS-CoV-2, in wastewater using high-throughput, highly multiplexed digital array PCR technology

使用高通量、高度多重数字阵列 PCR 技术提高废水中病原体检测（包括 SARS-CoV-2）的可扩展性、灵敏度和可解释性

• 批准号: 10264252
• 负责人: Rachel Todd Noble
• 金额: $ 99.75万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10264252
• 关键词: 2019-nCoV; Address; Biological Assay; Blood Circulation; Businesses; COVID-19; COVID-19 pandemic; Clinical; Communicable Diseases; Communities; Consumption; Coronavirus; Data; Data Analyses; Development; Disease; Disease Surveillance; Education; Feces; Future; Geographic Locations; Geography; Goals; Health; Home; Hour; Human; Individual; Laboratories; Location; Methods; Molecular; Monitor; Pathogen detection; Patients; Performance; Plants; Play; Precipitation; Prevalence; Public Health; Quality Control; Reaction; Reporting; Research; Respiratory distress; Role; Sampling; Schools; Sewage; Source; System; Technology; Testing; Time; Vaccination; Vaccines; Virus; Vision; biomarker panel; cost; digital; emerging pathogen; gastrointestinal; genetic testing; health management; health warning; improved; method development; molecular marker; multiplex assay; novel; novel coronavirus; pandemic disease; pathogen; research clinical testing; respiratory; response; tool; transmission process; viral RNA; wastewater epidemiology; wastewater monitoring; wastewater samples

PROJECT ABSTRACT Presently, the application of molecular technology such as RT-qPCR and digital PCR (dPCR) to quantify SARS-CoV-2 and related targets in wastewater is cumbersome, time consuming, and costly. While progress has been made on the development of methods and the interpretation of data, much remains to be improved for the technology to be used as a public health management tool. A major drawback in the current approaches are 1) the lack of streamlined and consistent pre-analytical processing steps, 2) coverage across the relevant targets requires a high number of reactions (>20) from any single sample to provide quantitative information, and 3) a lack of vision on the development of a pathogen/marker panel, much like those used in clinical arenas, for interpretation of the data across different states, regions and nations. The goal of this project will be to successfully navigate these three limitations toward development of a public health warning system that is not dependent on clinical testing and has the ability to rapidly address novel pathogen threats in the future.

项目摘要 目前，应用RT-qPCR和数字PCR（dPCR）等分子技术来定量 废水中的 SARS-CoV-2 和相关目标是繁琐、耗时且昂贵的。尽管 在方法开发和数据解释方面已经取得了进展，但仍有许多工作要做 改进该技术作为公共卫生管理工具。的一个主要缺点是 目前的方法是 1) 缺乏简化且一致的预分析处理步骤，2) 覆盖相关目标需要任何单个样本进行大量反应 (>20) 提供定量信息，以及 3) 缺乏对病原体/标记物组开发的远见， 与临床领域中使用的非常相似，用于解释不同州、地区和地区的数据 国家。该项目的目标是成功克服这三个限制 开发不依赖于临床测试并具有能力的公共卫生预警系统 快速应对未来新的病原体威胁。