Conserved RNA Secondary Structures in three Betacoronaviruses: MHV, BCoV, and MERS-CoV

三种 β 冠状病毒的保守 RNA 二级结构：MHV、BCoV 和 MERS-CoV

基本信息

批准号：
9016635
负责人：
JULIAN L LEIBOWITZ
金额：
$ 19.94万
依托单位：
TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-15 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9016635
关键词：
3&apos Untranslated Regions 5&apos Untranslated Regions Animals Antiviral Therapy Biochemical Biological Models Cattle China Chiroptera Cis-Acting Sequence Coronavirus Coronavirus Infections Coupled Data Disease Outbreaks Domestic Animals Elements Enteral Epidemic Event Family Future Genetic Transcription Genome Goals Growth Higher Order Chromatin Structure Human Indium Length Liver diseases Lower Respiratory Tract Infection Lung diseases Methods Middle East Respiratory Syndrome Coronavirus Modeling Murine hepatitis virus Nidovirales Nucleotides Play Primer Extension RNA RNA Sequences RNA chemical synthesis Regulation Resolution Role SARS coronavirus Severe Acute Respiratory Syndrome Signal Transduction Structure Testing Upper Respiratory Infections Viral Genome Virus Virus Replication Wild Animals cis acting element genetic approach genomic RNA insight man member mortality nervous system disorder new therapeutic target next generation sequencing novel pathogen prevent public health relevance research study respiratory reverse genetics small molecule

项目摘要

DESCRIPTION (provided by applicant): The Betacoronavirus genus includes two viruses, SARS-CoV and MERS-CoV that emerged from animal reservoirs to produce severe pulmonary disease in man in 2002, and 2012, respectively. In this application we propose to study the RNA secondary structure of MERS-CoV and two related coronaviruses, mouse hepatitis virus (MHV) and bovine coronavirus (BCoV) with the goal of identifying and functionally characterizing novel conserved RNA secondary structures in the genomes of these viruses. In aim one we will employ a state-of- the-art, high throughput approach, SHAPE-MaP, to generate biochemical data that allows the creation of highly accurate models of RNA secondary structures of large RNAs, such as coronavirus genomes. Through comparison of the RNA secondary structures of the genomes of MHV, BCoV, and MERS-CoV we will seek previously undiscovered phylogenetically conserved RNA secondary structures that are strong candidates to play a functional role in viral replication. In aim 2 we will employ reverse genetic approaches to characterize the functional role in MHV and MERS-CoV replication of conserved secondary structures identified in aim 1.

描述（由申请人证明）：贝塔科罗纳病毒属包括两个病毒，SARS-COV和MERS-COV，这些病毒从动物储层中出现到2002年男子和2012年的男性疾病中，我们建议在此应用中进行敏感。 MERS-COV和两个相关冠状病毒的RNA二级结构，小鼠肝炎病毒（MHV）和牛冠状病毒d在功能上表征了新型的保守RNA二级结构，该病毒的基因组，高通量方法，以产生生物化学数据。这允许建立大型RNA的RNA含量结构的高度精确度，例如冠状病毒基因组。候选者o在AIM 2中发挥功能作用。