Lipidomics of meconium in neurodevelopment

胎便在神经发育中的脂质组学

基本信息

批准号：
9090727
负责人：
Nathaniel W. Snyder
金额：
$ 23.48万
依托单位：
DREXEL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-04-22 至 2018-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9090727
关键词：
Address Androgens Autistic Disorder Behavior Biological Markers Blood Brain Brain region Carbon Chemical Structure Chemicals Child Child health care Clinical assessments Cohort Studies Communication Complex DNA Methylation Defecation Defect Development Elderly Ensure Environment Epigenetic Process Etiology Event Feces Folic Acid Future General Population Goals Head Health Human Infant Infection Inflammatory Response Institutes Investigation Knowledge Laboratories Light Lipids Meconium Mediator of activation protein Metabolism Methods Modification Molecular Genetics Morphologic artifacts Neurodevelopmental Disorder Newborn Infant Outcome Oxidative Stress Placenta Positioning Attribute Pregnancy Prevalence Research Resolution Resources Risk Risk Factors Sampling Science Social Interaction Source Spottings Steroids Structure Technology Testing Time Umbilical Cord Blood Unsaturated Fats Validation Work analytical method autism spectrum disorder base biomarker panel cohort developmental disease disease classification disorder control disorder risk experience feeding fetal histone methylation in utero instrument interest liquid chromatography mass spectrometry male maternal serum method development methyl group neurodevelopment oxidized lipid prenatal prenatal influence prospective public health relevance repetitive behavior research study trait

项目摘要

DESCRIPTION (provided by applicant): Lipids are critical to healthy neurodevelopment, are variably enriched across regions of the brain, and are integral to human metabolism. Meconium, the first bowel movements of a newborn, begin accumulation in approximately the 12th week of gestation, and contains high lipid content. Thus, meconium may provide a window into the abnormal neurodevelopment that occurs in the early etiology of autism spectrum disorder. This proposal will quantify and compare the lipid content of meconium in typically developing versus neurodevelopmentally delayed children from a prospective enriched risk cohort of early events in autism spectrum disorder. To extend the relevance of the putatively identified lipid biomarkers, the structures of the biomarkers will be elucidated, and the lipid content of the samples derived from the cohort will be compared to meconium from healthy newborns as well as other biospecimens from newborns. Finally, targeted methods will be developed in suitable matrices for future validation of the biomarkers. We will conduct this research in three specific aims as follows; Aim 1. Identify unknown chromatographic features with differential abundance between ASD and controls in a prospective enriched risk cohort. Aim 2. Structurally elucidate the putative biomarkers of ASD and compare the lipid content of meconium, placenta, and cord blood. Aim 3. Develop and validate a targeted method for the quantitation of putative biomarkers of ASD.

描述（由适用提供）：脂质对于健康的神经发育至关重要，在大脑的区域中富含富集，并且是人类代谢不可或缺的。胎粪是新生儿的第一个排便运动，大约在妊娠的第12周开始积聚，并含有高脂质含量。这是胎粪可以为自闭症谱系障碍早期病因的神经发育异常的神经发育提供一个窗口。提案将在自闭症谱系障碍中的前瞻性富集风险队列中量化和比较次胎胎儿和神经发育延迟的儿童的脂质含量。为了扩展推测已鉴定的脂质生物标志物的相关性，将阐明生物标志物的结构，并将来自同类的样品的脂质含量与来自健康的新生儿以及其他新生儿的生物素的胎粪进行比较。最后，将在适当的物品中开发有针对性的方法，用于将来验证生物标志物。我们将以以下三个特定目标进行这项研究； AIM 1。确定未知的色谱特征，在预期富集的风险队列中，ASD和对照之间具有差异的丰度。 AIM 2。可以结构阐明ASD的假定生物标志物，并比较胎粪，斑点和脐带血的脂质含量。目标3。开发并验证定量ASD假定生物标志物的目标方法。