Pan Coronavirus Genomic Surveillance of a Large NHP Colony

大型 NHP 群体的泛冠状病毒基因组监测

基本信息

批准号：
10575848
负责人：
Nicholas James Maness
金额：
$ 8.75万
依托单位：
TULANE UNIVERSITY OF LOUISIANA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-11-14 至 2024-10-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10575848
关键词：
2019-nCoV Amino Acid Sequence Homology Animals Birds Blood COVID-19 COVID-19 impact COVID-19 pandemic COVID-19 severity Camels Canis familiaris Case Fatality Rates Cessation of life Chiroptera Collection Common Cold Coronavirus Coronavirus Infections Data Disease Elements Exhibits Family Family suidae Felis catus Frequencies Future Genomics Health Human Immune Immunity Incidence Infection Macaca Macaca mulatta Middle East Respiratory Syndrome Coronavirus Modeling Molecular Nature Nose Outcome Phylogenetic Analysis Play Primates Publishing Rectum Research Research Project Grants Role SARS coronavirus Sampling Seasons Serology Site Swab Techniques Viral Virus Zoonoses betacoronavirus coronavirus disease cross reactivity epidemic potential human coronavirus interest next generation sequencing nonhuman primate novel novel coronavirus novel virus pandemic disease pandemic potential rectal response screening seasonal coronavirus vaccine evaluation zoonotic coronavirus

项目摘要

Project Summary/Abstract The novel coronavirus that emerged in late 2019, termed SARS-CoV-2, quickly spread throughout the world and has killed millions. A critical aspect of this pandemic that remains understudied is the impact of immunity to seasonal coronaviruses on COVID-19 disease. Several lines of evidence suggest there is an impact but the nature of that impact remains unclear. We have preliminary data confirming our colony of 5,000 rhesus macaques harbors a large fraction of animals with preexisting immunity to coronaviruses of unknown origin. This immunity may be important in the use of these animals for COVID-19 and other coronavirus disease. We hypothesize that our colony seasonally circulates human seasonal coronaviruses but we need to identify the actual viruses circulating. Here, we will use multiple approaches to assess the frequency of these infections and to identify the actual viruses. In Aim 1, we will use pan-coronavirus primers to quantify coronavirus infections in our colony. We will sample both nasal and rectal sites as coronaviruses are known to be shed from both. In Aim 2, we will use next generation sequencing to identify the viruses circulating. We will apply phylogenetic approaches to characterize the diversity of the virus or viruses in our colony. These data will have important implications for the use of macaques for coronavirus infections in general and may shed light on the coronavirus family.

项目概要/摘要 2019 年底出现的新型冠状病毒（称为 SARS-CoV-2）迅速在全球传播并杀死了数百万人。此次大流行的一个尚未得到充分研究的关键方面是免疫力的影响季节性冠状病毒对 COVID-19 疾病的影响。多项证据表明存在影响，但这种影响的性质仍不清楚。我们有初步数据证实我们的种群有 5,000 只恒河猴猕猴体内有很大一部分对来源不明的冠状病毒具有预先存在的免疫力。这种免疫力对于使用这些动物治疗 COVID-19 和其他冠状病毒疾病可能很重要。我们假设我们的殖民地季节性传播人类季节性冠状病毒，但我们需要确定实际传播的病毒。在这里，我们将使用多种方法来评估这些感染的频率并识别真正的病毒。在目标1中，我们将使用泛冠状病毒引物来量化冠状病毒我们殖民地的感染。我们将对鼻腔和直肠部位进行采样，因为已知冠状病毒会脱落来自两者。在目标 2 中，我们将使用下一代测序来识别正在传播的病毒。我们将申请系统发育方法来表征我们群体中病毒的多样性。这些数据会有对使用猕猴治疗冠状病毒感染具有重要意义，并可能有助于阐明冠状病毒家族。