Molecular Interactions and Imaging Core

分子相互作用和成像核心

基本信息

批准号：
10271748
负责人：
HENRY N HIGGS
金额：
$ 44.63万
依托单位：
DARTMOUTH COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-15 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10271748
关键词：
Actins Adopted Area Award Awareness Back Binding Biochemical Biological Assay Biology Calorimetry Cells Centers of Research Excellence Charge Collaborations Collection Communities Consultations Core Facility Cryoelectron Microscopy Custom DNA Repair Data Data Analyses Development Drosophila genus Electron Microscopy Electron Microscopy Facility Embryo Endocytosis Ensure Environment Equipment Experimental Designs Extramural Activities Faculty Fluorescence Microscopy Funding Goals Grant Histone H3 Human Image Image Analysis Imaging technology In Situ In Vitro Individual Influenza Hemagglutinin Infrastructure Institutes Institution Laboratories Lead Location Mammalian Cell Mass Spectrum Analysis Measures Melanoma Cell Methods Microscopy Mission Modeling Molecular Molecular Structure Monitor Monoclonal Antibodies Norris Cotton Cancer Center Nuclear Organelles Participant Phase Pilot Projects Play Population Positioning Attribute Process Production Program Reviews Protein Dynamics Protein Isoforms Proteins Proteomics Recovery Research Research Infrastructure Research Personnel Research Project Grants Resolution Resources Role Scientist Services Specialist Spectrum Analysis Strategic Planning Structure Surface Plasmon Resonance Surveys T-Lymphocyte Talents Techniques Technology Time Titrations Training Validation Virus Water Work Workload base biophysical properties biophysical techniques cell motility cellular imaging cost cost effective design experimental study flexibility fluorescence microscope imaging study improved instrument instrumentation interest intravital imaging light microscopy member method development molecular imaging operation polymerization pressure recruit success tool tumor

项目摘要

The Molecular Interactions and Imaging Core (MIIC) increases the rigor and sophistication of bioMT research projects by facilitating the use of molecular binding assays and imaging technologies. One goal of the MIIC is to eliminate barriers to these technologies and provide advice in the optimal collection and analysis of the data. In phase I of this grant, MIIC has centralized oversight of existing resources and participated in the acquisition of new equipment, both within the bioMT facility and in conjunction with two existing microscopy cores at Dartmouth. We have hired staff to provide training, guidance, and experimental support in the characterization of molecular interactions. We have also hired two imaging navigators to help investigators with experimental design, image acquisition, and image analysis. These activities significantly enhanced the ability of our phase I research project leaders (RPLs) and all bioMT investigators to conduct quantitative binding and imaging studies at Dartmouth, and thus contributed to their extramural and academic success. Our plans for the next funding period have been guided by an inclusive and intensive program review process. In phase II, MIIC has four specific aims. Specific Aim 1 is to provide customized support to help the four RPLs attain their research goals and achieve independence. As active participants in the development of the four research projects, the director and research navigators engaged with the RPLs to comprehensively assess the methods required by each. From this survey we identified key technologies to quantify binding interactions, to visualize molecular locations and dynamics of proteins and organelles in cells, and to assess distribution of cells within tumors in situ. Specific Aim 2 is to expand our services to three key areas: mass spectrometry/proteomics, higher resolution cellular imaging by super-resolution microscopy and electron microscopy, and cryo-electron microscopy analysis of macromolecular structure. In all three areas, MIIC will continue the cost-effective practice of leveraging existing equipment at Dartmouth and elsewhere to accomplish these goals. Specific Aim 3 is to extend support for image analysis, which is an increasingly pressing need for many investigators. Specific Aim 4 prepares for sustainability of the Core in phase 3. The MIIC works closely with the Molecular Tools Core, which is housed in the same, newly renovated bioMT Core Facilities Suite. In addition, the MIIC has established strong working relationships with existing cores at Dartmouth (including several supported by other COBRE awards) as well as at other IDeA partner institutions. Within all four aims, the MIIC has been designed to adapt flexibly to technological needs that emerge as bioMT investigators make scientific progress. The MIIC will also share best practices and ensure up-to-date training for all bioMT investigators interested in monitoring, and in most cases quantifying, binding interactions of their biomolecules, whether as purified components or within cells. It will thus directly support all four bioMT Research Projects as well as affiliated bioMT faculty, and will enhance the research infrastructure available at Dartmouth and among our regional IDeA partners.

分子相互作用和成像核心 (MIIC) 提高了 bioMT 研究的严谨性和复杂性通过促进分子结合测定和成像技术的使用来开展项目。 MIIC 的目标之一是消除这些技术的障碍，并提供最佳数据收集和分析的建议。在本次赠款的第一阶段，MIIC集中监管现有资源并参与收购的新设备，既在 BioMT 设施内，又与两个现有的显微镜核心相结合达特茅斯。我们聘请了工作人员来提供表征方面的培训、指导和实验支持分子相互作用。我们还聘请了两名成像导航员来帮助研究人员进行实验设计、图像采集和图像分析。这些活动显着增强了我们第一阶段的能力研究项目负责人 (RPL) 和所有 bioMT 研究人员进行定量结合和成像研究在达特茅斯，从而为他们的校外和学术成功做出了贡献。我们的下一次融资计划期间以包容性和深入的方案审查进程为指导。在第二阶段，MIIC有四个具体目标。具体目标 1 是提供定制支持，帮助四个 RPL 实现其研究目标并实现独立。作为四个研究项目开发的积极参与者，主任研究导航员与 RPL 合作，全面评估每个项目所需的方法。从这项调查中，我们确定了量化结合相互作用、可视化分子位置的关键技术以及细胞中蛋白质和细胞器的动态，并原位评估肿瘤内细胞的分布。具体的目标 2 是将我们的服务扩展到三个关键领域：质谱/蛋白质组学、更高分辨率的细胞通过超分辨率显微镜和电子显微镜成像以及冷冻电子显微镜分析大分子结构。在所有三个领域，MIIC 将继续利用现有的具有成本效益的做法达特茅斯和其他地方的设备来实现这些目标。具体目标 3 是扩展对图像的支持分析，这对于许多研究人员来说是越来越迫切的需要。具体目标 4 为可持续发展做好准备第三阶段的核心。MIIC 与分子工具核心密切合作，后者位于相同的、新装修的 BioMT 核心设施套件。此外，MIIC 还建立了牢固的工作关系与达特茅斯（包括其他 COBRE 奖项支持的几个）以及其他 IDeA 的现有核心合作机构。在所有四个目标中，MIIC 旨在灵活适应技术需求随着 BioMT 研究人员取得科学进步而出现。 MIIC 还将分享最佳实践并确保为所有对监测以及在大多数情况下量化、结合感兴趣的 BioMT 研究人员提供最新培训它们的生物分子之间的相互作用，无论是作为纯化成分还是在细胞内。因此它将直接支持所有四个bioMT研究项目以及附属的bioMT教师，并将加强研究基础设施可在达特茅斯学院和我们的地区 IDeA 合作伙伴中获取。