Core B: Animal Core and Germ-Free Mouse Core

核心B：动物核心和无菌小鼠核心

基本信息

批准号：
10588796
负责人：
Lucy Hoffmann Kennedy
金额：
$ 11.44万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-02-01 至 2028-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10588796
关键词：
Accreditation Adherence American Animal Experimentation Animals Behavior Blood Body Weight Caring Certification Consultations Derivation procedure Diabetes Mellitus Diet Dose Ensure Environmental Monitoring Experimental Designs Fasting Feces Film Genetic Engineering Germ-Free Glucose Gnotobiotic Goals Guidelines Health Health Status Housing IACUC Inbred BALB C Mice Institution Laboratories Laboratory Animal Medicine Laboratory mice Maintenance Michigan Modeling Monitor Mus Obesity Personal Satisfaction Phenotype Play Process Protocols documentation Quarantine Reproducibility Research Research Personnel Role Services Techniques Technology Training United States Department of Agriculture Universities Urine Veterinarians Workplace animal care college flexibility germ free condition metabolic phenotype pathogen sample collection segregation ventilation

项目摘要

Project Summary / Abstract – Animal Care and Germ-Free Mouse Core The Animal Care and Germ-Free Mouse Core of the Michigan MPMOD will play a central role in the management of animal flow through the Center’s MPB Phenotyping Core from initial acquisition to final disposition of imported mice. Both the Core Director and Associate Director are veterinarians and diplomates of the American College of Laboratory Animal Medicine. Specific functions of the Animal Care and Germ-Free Mouse Core include the coordination of mouse importation, quarantine, and environmental testing for a panel of murine pathogens, as well as providing housing, husbandry, and veterinary care of mice in accordance with the MPMOD (Administrative Core) guidelines. Research services such as hands-on technical support and germ-free technology and expertise will be unique components of our Core. Germ-free C57BL/6J, C57BL/6NCrl, BALB/c, and Swiss-Webster mice as well as several genetically engineered strains will be available to MPMOD investigators. In addition, investigators may import germ-free mice from other institutions or request rederivation of new germ-free strains. Experimental manipulations available for germ-free and SPF mice include routine treatments, sample collections, and in-house analysis (blood, feces, urine, etc.), training, and experimental design consultation, among others. Goals of the Animal Care and Germ-Free Mouse Core are to provide the highest level of health and well-being as well as veterinary expertise for all research animals in our USDA-certified and AAALAC-accredited facilities. All Core services will be performed under University of Michigan IACUC approved protocols. All SPF mice will be housed in Allentown ventilated racks. Germ-free or gnotobiotic mice will be maintained in flexible-film isolators or Tecniplast Isocage racks. Barrier conditions including cage changing in vertical laminar flow changing stations, strict adherence to microisolator techniques, and use of isolated rooms for colony segregation will assure maintenance of pathogen-free animals. The Animal Core will also provide oversight for technical support such as dose administration, obtaining body weights, fasting, glucose levels, providing and monitoring special diets, and transfer of mice to and from ULAM housing to the laboratories of the MPB Phenotyping Core.

项目摘要/摘要——动物护理和无菌小鼠核心密歇根 MPMOD 的动物护理和无菌小鼠核心将在通过中心的 MPB 表型核心管理从最初采集到最终的动物流程核心主任和副主任都是兽医和外交官。美国实验动物医学学院的动物护理和无菌的具体功能。 Mouse Core 包括协调小鼠进口、检疫和小组环境测试鼠病原体，并根据规定为小鼠提供住房、饲养和兽医护理 MPMOD（管理核心）研究服务，例如实践技术支持和指南。无菌技术和专业知识将成为我们无菌 C57BL/6J 核心的独特组成部分。 C57BL/6NCrl、BALB/c 和 Swiss-Webster 小鼠以及几种基因工程品系将被此外，研究人员还可以从其他机构进口无菌小鼠。或请求重新衍生新的无菌菌株，可用于无菌和 SPF。小鼠包括常规治疗、样本采集、内部分析（血液、粪便、尿液等）、训练、动物护理和无菌小鼠核心的目标和实验设计咨询等。为所有研究动物提供最高水平的健康和福祉以及兽医专业知识在我们经过美国农业部认证和 AAALAC 认证的设施中，所有核心服务都将在大学下进行。密歇根州 IACUC 批准的方案。所有 SPF 小鼠都将饲养在无菌或无菌的架子上。无菌小鼠将被饲养在柔性薄膜隔离器或 Tecniplast Isocage 架上。包括在垂直层流更换站更换笼子，严格遵守微隔离器技术，使用隔离室进行群体隔离将确保维持无病原体的动物。 Animal Core 还将对技术支持进行监督，例如剂量管理、获取尸体体重、禁食、血糖水平、提供和监测特殊饮食以及将小鼠转移至 ULAM 或从 ULAM 转移 MPB 表型核心实验室的住房。