Purchase of a Multi-Angle Light Scattering System with Integrated Size Exclusion Chromatography

购买具有集成尺寸排阻色谱法的多角度光散射系统

• 批准号: 9075386
• 负责人: Rachel Wagner Martin
• 金额: $ 13.86万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075386
• 关键词: Binding Proteins; Biomedical Research; Biomimetic Materials; Cataract; Chlamydia; Data; Diagnosis; Disease; Eye Lens Protein; Faculty; Future; Gastric ulcer; Medical; Membrane Proteins; Minor; Molecular Sieve Chromatography; Research; Research Personnel; System; Technology; United States National Institutes of Health; base; biophysical chemistry; design; innovation; instrument; light scattering; macromolecule; malignant stomach neoplasm; nanoparticle; novel; programs; protein folding; structural biology

 DESCRIPTION (provided by applicant): The proposed multi-angle light scattering instrument with integrated size exclusion chromatography (SEC-MALS). The instrument will support five major users, all of whom are engaged in biomedical research, and four of whom are supported by NIH. Specific projects are focused on the following: determining the structural aggregation propensity and oligomerization state of structural eye lens proteins, understanding intermediates formed during protein folding, characterizing the interactions of membrane proteins with both membranes and protein binding partners, designing novel biomimetic materials and nanoparticles for medical diagnosis. The macromolecules studied in these projects are associated with numerous diseases, such as cataract, chlamydia, gastric ulcers and cancer. Additionally, three minor users, all of whom are junior faculty with promising research programs, will have the opportunity to use this instrument, advancing their research and strengthening their future proposals. The biophysical information and understanding gained with the proposed instrument could provide the basis for designing new, innovative treatments. The biggest impact of the proposed system derives from the fact that there is currently no modern, functional MALS instrument available for users at UCI. As a result, researchers who need this technology must rely on external collaborators and companies, slowing down the pace of research progress and severely curtailing the amount of data that can be collected. The Major and Minor Users will all benefit from the DLS and SLS capabilities of the new instrument, enabling us to make a major impact on the fields of structural biology, biomimetic materials, and biophysical chemistry. The acquisition of the proposed SEC-MALS instrument is essential to alleviating UCI's current and future characterization demands and for UCI to continue doing high-impact biomedical research.

 描述（由适用提供）：具有集成尺寸排除色谱法（SEC-MALS）的拟议的多角度光散射仪器。该工具将为五个主要用户提供支持，他们全部从事生物医学研究，其中四个得到了NIH的支持。特定的项目集中在以下内容上：确定结构眼镜蛋白的结构聚集承诺和寡聚状态，了解在蛋白质折叠过程中形成的中间体，表征膜蛋白与膜蛋白与蛋白质结合伙伴的相互作用，从而设计新的生物含量材料和Nanaparticles进行医学诊断。这些项目中的大分子研究与许多疾病有关，例如白内障，衣原体，胃溃疡和癌症。另外三个未成年人，他们都是有希望的研究计划的初级教师，他们将有机会使用该工具，推进他们的研究并加强他们的未来建议。拟议的仪器获得的生物物理信息和理解可以为设计新的创新治疗方法提供基础。拟议系统的最大影响源于以下事实：目前尚无UCI用户可用的现代功能性MALS仪器。结果，需要此技术的研究人员必须依靠外部合作者和公司，从而降低了研究进度的步伐，并严重减少了可以收集的数据量。主要使用者和未成年人都将从新仪器的DLS和SLS功能中受益，从而使我们能够对结构生物学，仿生材料和生物物理化学的领域产生重大影响。拟议的SEC-MALS工具的获取对于减轻UCI当前和未来的特征要求以及UCI继续进行高影响力的生物医学研究至关重要。