Core C: Comparative Pathology Core

核心 C：比较病理学核心

基本信息

批准号：
10573310
负责人：
Enrico Radaelli
金额：
$ 27.89万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-02-15 至 2027-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10573310
关键词：
Address Algorithms Animal Experiments Animal Model Animals Archives Autopsy Biological Biological Markers Biological Process Board Certification Canis familiaris Carbon Cells Classification Clinical Collaborations Collection Comparative Pathology Computer software Consultations Data Dedications Development Ensure Epithelium Evaluation Fibrosis Goals Growth Hematoxylin and Eosin Staining Method Histological Techniques Histology Histopathology Image Analysis Immune Immunofluorescence Immunologic Immunohistochemistry In Situ Hybridization Inflammation Inflammatory Infrastructure Label Laboratories Lesion Macrophage Malignant - descriptor Measurable Modality Modernization Molecular Morphology Mus Normal tissue morphology Outcome Paraffin Embedding Pathologic Pathologist Pathology Patients Phenotype Play Population Pre-Clinical Model Preparation Procedures Protocols documentation Protons Radiation therapy Reproducibility Research Design Research Personnel Rodent Role Route Sampling Services Severities Signal Pathway Sirius Red F3B Slide Solid Specimen Stains Standardization Structure System T-Lymphocyte Techniques Technology Testing Therapeutic Index Time Tissue Sample Tissues Toxic effect Training Translations Trichrome stain method Variant Work animal tissue cytokine digital imaging digital pathology efficacy evaluation experience functional status immune cell infiltrate immunological status interest neoplastic neoplastic cell neutrophil particle pathology imaging personalized approach pre-clinical preclinical study preservation programs response tissue preparation tool translational study tumor tumor microenvironment whole slide imaging

项目摘要

Project Summary The study of the preclinical animal models included in all four Projects plays a critical role to investigate the overall hypothesis that Proton/Carbon Particle FLASH Radiotherapy is superior to Standard Particle Radiotherapy in protecting normal tissues while maintaining equipotent malignant growth control. The extrapolation of robust preclinical data in these experimental settings relies heavily on the unbiased assessment of specific histopathological parameters to investigate the biological effects of FLASH particle radiotherapy as compared to Standard particle radiotherapy on designated neoplastic lesions and adjacent normal tissues. The Comparative Pathology Core (CPC; Core C) will provide its expertise contributing to the planning, evaluation, and interpretation of the pathology endpoints of the animal experiments included in the Projects. As a PennVet clinical laboratory, Core C importantly follows standardized working protocols with strict QA/QC procedures. The histopathology service offered by Core C will ensure accurate collection, proper preservation, timely processing, and staining of the animal specimens. Core C will provide the necessary technical support and submissions of tissue samples will follow a prioritized route. In addition to standard histopathology, Core C will also develop and validate tailored approaches to investigate and quantify tissue changes specifically associated with RT such as fibrosis, intralesional distribution of inflammatory/immune cell populations, expression of markers to evaluate epithelial barrier integrity, etc. The board-certified veterinary pathologists of Core C will deliver expert evaluation and unbiased interpretation of the pathology endpoints. Objective and reproducible quantification of tissue changes in response to the diverse RT modalities will be guaranteed by the application of validated scoring systems and the utilization of software-based algorithms for digital pathology and image analysis. Core pathologists have advanced training in digital imaging pathology from Leica pathology systems. Moreover, Dr. Assenmacher has collaborated with Leica in the development of analysis tools, lending him particular experience in the analytic modules of the digital pathology system.

项目概要所有四个项目中包含的临床前动物模型的研究对于研究质子/碳粒子闪光放射治疗优于标准粒子的总体假设放射治疗可以保护正常组织，同时保持同等的恶性生长控制。这在这些实验环境中对可靠的临床前数据进行推断在很大程度上依赖于无偏见的评估特定组织病理学参数以研究 FLASH 颗粒的生物学效应与标准粒子放射治疗相比，对指定的肿瘤病变和邻近的放射治疗正常组织。比较病理学核心（CPC；核心 C）将提供其专业知识，为计划、评估和解释动物实验的病理学终点项目。作为 PennVet 临床实验室，Core C 重要的是遵循严格的标准化工作协议质量保证/质量控制程序。 Core C提供的组织病理学服务将确保准确采集、正确动物标本的保存、及时处理和染色。核心C将提供必要的技术支持和组织样本的提交将遵循优先顺序。除了标准的组织病理学方面，Core C 还将开发和验证定制的方法来研究和量化组织与放疗特别相关的变化，例如纤维化、炎症/免疫细胞的病灶内分布种群、评估上皮屏障完整性的标记物表达等。经过委员会认证的兽医 Core C 的病理学家将对病理学终点进行专家评估和公正的解释。响应不同 RT 模式的组织变化的客观且可重复的量化将是通过应用经过验证的评分系统和基于软件的算法来保证用于数字病理学和图像分析。核心病理学家接受过数字成像病理学高级培训来自徕卡病理系统。此外，Assenmacher 博士还与徕卡合作开发分析工具的使用，使他在数字病理系统的分析模块方面拥有特殊的经验。