Molecularly imprinted nanoparticles as new tools to elucidate T cell signaling events

分子印迹纳米颗粒作为阐明 T 细胞信号传导事件的新工具

基本信息

批准号：
10559701
负责人：
AMY H ANDREOTTI
金额：
$ 19.13万
依托单位：
IOWA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-02-01 至 2024-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10559701
关键词：
Adaptor Signaling Protein Address Affinity Amino Acid Sequence Autoimmunity Binding Binding Sites Biological Assay CD3 Antigens CRISPR/Cas technology Cell membrane Cell physiology Cells Cellular biology Chemistry Collaborations Development Disease Elements Event Exhibits Family Generations Genes Goals Growth ITAM Immune Immune response Immunosuppression In Vitro Investigation Investments Knowledge LCP2 gene Masks Mediating Methods Micelles Molecular Molecular Target Morphologic artifacts Mutagenesis Mutation PXXP Motif Pathway interactions Permeability Phosphorylation Phosphorylation Site Phosphotransferases Phosphotyrosine Post-Translational Modification Site Post-Translational Protein Processing Preparation Prevalence Proline Proline-Rich Domain Proteins Proteome Protocols documentation Reagent Recurrence Research Design Signal Pathway Signal Transduction Site Specificity Surface T-Lymphocyte Technology Testing Toxic effect Tyrosine Water cell type combinatorial crosslink efficacy testing experimental study imprint in vitro testing knockout gene molecular site nanoimprinting nanoparticle non-Native novel strategies novel therapeutics performance tests polyproline prevent protein aminoacid sequence protein complex protein protein interaction public health relevance receptor receptor function structural biology tool

项目摘要

Project Summary The study of cellular signaling has benefitted greatly from existing approaches to alter signaling pathways in a controlled manner to delineate specific molecular events that mediate cell function. Mutagenesis is one such method used to remove a specific recognition site, or site of post- translational modification, to evaluate signaling consequences and deduce the importance of the target site. While powerful, this approach requires cell development, growth, and activation to take place in the context of a non-native gene which can have ancillary effects confounding the experimental results. The submitted application takes a completely new approach to the study of cellular signaling; molecularly imprinted nanoparticles (MINPs) have been developed by Yan Zhao that exhibit exquisite selectivity and affinity for their short target sequences. Cell permeability has been demonstrated and in this application Zhao and Andreotti will test the efficacy of these new reagents in the study of T cell signaling. Specific target sequences are chosen for MINP generation, binding affinity and specificity will be tested in vitro and in cell lysates, and internalization and target binding of MINPs in T cells will be fully characterized. If successful, these proof of principle experiments will pave the way for application of MINP technology to every corner of the cell signaling field.

项目概要细胞信号传导的研究极大地受益于现有的改变信号传导的方法以受控方式描绘介导细胞功能的特定分子事件的途径。诱变是一种用于去除特定识别位点或后位点的方法。翻译修饰，评估信号转导结果并推断其重要性目标站点。这种方法虽然强大，但需要细胞发育、生长和激活发生在非天然基因的背景下，可能会产生混淆的辅助效应实验结果。提交的申请采用了一种全新的方法来研究细胞信号传导； Yan 开发了分子印迹纳米颗粒 (MINP) 赵对短目标序列表现出精湛的选择性和亲和力。细胞渗透性已得到证明，在此应用中，Zhao 和 Andreotti 将测试这些新试剂在 T 细胞信号传导研究中的功效。具体靶序列为选择用于 MINP 生成，将在体外和细胞内测试结合亲和力和特异性裂解物、T 细胞中 MINP 的内化和靶标结合将得到全面表征。如果成功了，这些原理验证实验将为 MINP 的应用铺平道路技术渗透到细胞信号传导领域的各个角落。