Generating new mouse mutant strains

产生新的小鼠突变品系

• 批准号: 10266648
• 负责人: Karl Eric Pfeifer
• 金额: $ 81.82万
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10266648
• 关键词: CRISPR/Cas technology; ES Cell Line; Embryo; Enhancers; Epigenetic Process; Gene Expression Regulation; Genome Stability; Goals; Hematopoiesis; Immune system; Implant; Injections; Intramural Research Program; Iron; Malaria; Modeling; Mutant Strains Mice; Mutate; National Institute of Child Health and Human Development; Operative Surgical Procedures; Pathway interactions; Protocols documentation; RNA; Research; Research Personnel; Research Support; Risk; Scientist; Signal Pathway; T-Cell Development; Thymus Gland; United States National Institutes of Health; Work; blastocyst; design; embryonic stem cell; immune function; mouse model; novel; stem cell biology; zygote

During the last year our research core has worked with ten NIH research units to generate novel embryonic stem cell lines and new mouse models (8 projects, including 6 CRISPR/Cas9 Zygote injections). This research included establishment of models to study signal pathway interactions in the immune system, T cell development, hematopoiesis, stem cell biology, epigenetic inheritance in the early embryo, mechanisms regulating genomic stability, RNA metabolis, gene regulation and enhancer function, and iron methabolism and malaria risk. We also performed specialized surgeries including thymus implants to support research on immune function.

去年，我们的研究核心与 10 个 NIH 研究单位合作，生成新型胚胎干细胞系和新的小鼠模型（8 个项目，包括 6 个 CRISPR/Cas9 Zygote 注射）。该研究包括建立模型来研究免疫系统中信号通路相互作用、T细胞发育、造血、干细胞生物学、早期胚胎表观遗传、基因组稳定性调节机制、RNA代谢、基因调控和增强子功能以及铁代谢和疟疾风险。我们还进行了专门的手术，包括胸腺植入，以支持免疫功能的研究。